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AcSDKP抑制体外培养条件下人骨髓间充质干细胞的增殖

戴国, 黄畅, 李烨, 皮亦华, 汪保和

湖南师范大学生命科学学院.湖南,长沙 410081

摘要

N--乙酰基--丝氨酰--天冬氨酰--赖氨酰--脯氨酸（N--acetyl--seryl--aspartyl--lysyl--proline,AcSDKP）是一种具有生理调控活性的四肽因子，对造血干，祖细胞增殖具有抑制作用。该研究采用集落形成实验、甲基偶氮唑盐（MTT）比色法、细胞分裂指数测定等方法，考察了AcSDKP对体外培养的人骨髓间充质干细胞（mesenchymal stem cell，MSC）增殖的影响。结果显示，在AcSDKP浓度为1×10~(-12) mol／L---1×10~(-9) mol／L的培养体系中，人骨髓MSC集落生成率和大小、活力细胞数和分裂指数均降低，最大效应浓度为1×10~(-11)mol／L。以上实验结果表明，在体外培养条件下，一定浓度的AcSDKP对人骨髓MSC的增殖具有抑制作用。

关键词： AcSDKP; 增殖; 抑制; 间充质干细胞; 骨髓

Inhibitory effects of AcSDKP on proliferation of human bone marrow mesenchymal stem cells {sl in vitro}

Dai Guo, Huang Chang, Li Ye, Pi Yihua, Wang Baohe

College of Life Sciences, Hunan Normal University.Changsha 410081,Hunan

Abstract

The tetrapeptide N-acetyl-seryl-aspartyl-lysyl-proline （AcSDKP）, a physiological regulator of cell proliferation, has been principally reported as a potent inhibitor of the proliferation of haematopoietic stem cells and progenitors. The purpose of this study was to investigate whether the AcSDKP may directly affect the proliferative potential of human bone marrow mesenchymal stem cells （MSCs） in vitro. We added AcSDKP to the cultures of human bone marrow mononuclear cells and measured the number and average area of MSC colonies. MTT colorimetric assay and mitotic index determination were further used to examine the proliferative state of the third passage MSCs in subcultures with or without the addition of AcSDKP. In addition, we evaluated whether AcSDKP may kill MSCs by the trypan blue dye exclusion test. The results showed that the colony forming capacity, the number of viable cells and the mitotic index were reduced in human bone marrow MSCs cultured in 1×10~(-12) mol/L to 1×10~(-9) mol/L AcSDKP. Maximum inhibitory activity appeared in 1×10~(-11)mol/L of AcSDKP. No difference in percent of living cells was observed between the MSC subcultures with and without the addition of AcSDKP. As a result, AcSDKP within a certain range of concentrations has negatively regulatory effects on the proliferation of human bone marrow MSCs in vitro.

Key words: AcSDKP;Proliferation;Inhibition;mesenchymal stem cell;bone marrow

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引用本文：

戴国, 黄畅, 李烨, 皮亦华, 汪保和. AcSDKP抑制体外培养条件下人骨髓间充质干细胞的增殖[J]. 生理学报 2006; 58 (2): .

Dai Guo, Huang Chang, Li Ye, Pi Yihua, Wang Baohe. Inhibitory effects of AcSDKP on proliferation of human bone marrow mesenchymal stem cells {sl in vitro}. Acta Physiol Sin 2006; 58 (2): (in Chinese with English abstract).