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一氧化氮的释放对海马脑片CAl区痫样放电的影响

陆巍, 陈刚, 程介士

上海医科大学神经生物学教研室；上海医科大学化学教研室；医学神经生物学国家重点实验室

摘要

用自制的一氧化氮（NO）敏感电极──Nation-壳聚糖合镍修饰铂电极（Nation-CTS（Ni）-Pt）连续测定了青霉素致痫海马脑片CAl区锥体层神经元NO的释放，并同时观察了NO合酶抑制剂7-nitro-indaole（7-NI）及Nω-nitro-L-arginine（L-NNA）对诱发痫波及NO释放量的影响。研究观察到：（1）在青霉素致痫脑片模型上，诱发的痫波随青霉素浓度的增加而增多，与此同时，NO释放量亦增加并存在剂量反应关系；（2）NO合酶抑制剂7-NI及L-NNA抑制海马脑片CAl区NO的产生的同时，可部分翻转青霉素的致痫作用；（3）NO敏感电极检测线性范围为4．5×10－4～1．0×10－8mol／L，可用于生物组织如脑片的NO释放量测定。结果提示，NO可能有致痫作用；L-NNA及7-NI对癫痫有抑制作用；NO敏感电极的应用为生物体NO的连续实时测定提供了有效的实验工具。

关键词： 海马脑片诱发痫波; 一氧化氮; NO敏感电极; 7-NI L-NNA

Cui Hong

First Clinical Institute，China Medical University. Shenyang 110001,Liaoning, China

Abstract

Using a nitric chide (NO)-sensitive platinum microelectrode(SNM) modified withchitosan nickel (Ⅱ) complex and Nafion (Nation-CTS (Ni)-Pt), we observed the effectof nitric oxide synthase (NOS) inhibitor 7-nitro-indazole (7-NI) and N-nitro-L-arginine(L-NNA) on penicillin-treated hippocampal slices by simultaneously recording thestimulus-evoked field potentials and nitric oxide release from CA1 pyramidal neurons. Ourresults show: (1) The linear relationship range and detection limit of SNM were 4.5 ×10-4~ 1.0 ×10-8 mol/L and 5.0 × 10-8 mol/L respectively. (2) Penicillin (PEN)could elevate NO release, the number and amplitude of stimulus-evoked field potentialsspike (SEPS) in concentration-dependent manner; (3) Both 7-NI and L-NNA depressedNO release and partly reversed the effect of penicillin. The above findings suggest thatconvulsant provoking effect of NO could be inhibited by NOS inhibitors.

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引用本文：

陆巍, 陈刚, 程介士. 一氧化氮的释放对海马脑片CAl区痫样放电的影响[J]. 生理学报 1998; 50 (5): .

Cui Hong. . Acta Physiol Sin 1998; 50 (5): (in Chinese with English abstract).