过刊浏览

牛磺酸对急性低氧促培养的兔肺小动脉平滑肌细胞产生血栓素A_2和前列环素的影响

胡德辉

第一军医大学生理教研室

摘要

本工作观察了牛磺酸（taurine）对分离培养家兔肺小动脉平滑肌细胞（PASMCs）在急性低氧条件下培养液中血栓素B2（TxB2）和6-酮-前列腺素F1α（6-keto-PGF1α）含量及其比值变化的影响，同时用环加氧酶抑制剂吲哚美辛干预，以探讨牛磺酸的作用途径。结果是：低氧使PASMCs培养液中TxB2和6-keto－PGF1α含量及其比值显著增加。常氧和低氧条件下，终浓度为2．5×10-5mol／L的牛磺酸显著降低TxB2的含量；显著提高6-keto-PGF1α的含量，终浓度为2．5×10－3mol／L的吲哚美辛显著降低TxB2和6-keto-PGF1α含量，牛磺酸和吲哚美辛共同作用，TxB2的含量进一步降低，6－keto-PGF1α含量比单纯用吲哚美辛增加明显。这些结果表明，牛磺酸可能通过抑制PASMCs内的TxA2合成酶的活性和增强前列环素（PGI2）合成酶的活性拮抗低氧增强PASMCs产生TxA2和PGI2，使TxA2与PGI2的比值显著降低而逆转低氧性肺血管收缩。

关键词： 血栓素A_2; 前列环素; 牛磺酸; 肺小动脉平滑肌细胞; 吲哚美辛; 低氧

Cui Hong

First Clinical Institute，China Medical University. Shenyang 110001,Liaoning, China

Abstract

The results of the present study demonstrated that the concentrations of thromboxane B2 (TxB2a) and 6-keto-prostaglandin F1a. (6-keto-PGF1a), and their ratio were significantly increased in the medium of intra-pulmonary arteriolar smooth muscle cells (PASMCs) under acute, hypoxia. Under either normoxia or hypoxia the concentration of TxB2 was significantly decreased with addition of taurine, while that of 6-keto-PGF1a. was significantly increased. With the addition Of indomethacin, however, under similar conditions, the concentrations of both TxB2 and 6-keto-PGF1a. were decreased. Furthermore, the effect of indomathecin on TxA2 and 6-keto-PGF1a. concentrations could be revend by taurine.

Key words: ;;;;;

收稿日期：　　录用日期：

通讯作者：　　E-mail:

引用本文：

胡德辉. 牛磺酸对急性低氧促培养的兔肺小动脉平滑肌细胞产生血栓素A_2和前列环素的影响 [J]. 生理学报 1998; 50 (4): .

Cui Hong. . Acta Physiol Sin 1998; 50 (4): (in Chinese with English abstract).