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孤儿受体TR3在小鼠睾丸中的定位和表达

穆小民, 刘以训

中国科学院动物研究所生殖生物学国家重点实验室

摘要

本文采用原位杂交和免疫组织化学技术，观察孤儿受体TR3及其mRNA在小鼠睾丸中的表达及细胞定位。结果表明，在小鼠睾丸中有显著量的孤儿受体TR3mRNA和蛋白表达，其表达量在不同曲细精管有明显的差异；孤儿受体TR3蛋白主要定位于生精细胞，其mRNA在生精细胞特异表达，主要在精原细胞和发育早期的初级精母细胞表达，提示孤儿受体TR3在小鼠曲细精管精子发生的早期阶段中起着调控作用。

关键词： 孤儿受体TR3; 原位杂交; 免疫组织化学; 精子发生

Cui Hong

First Clinical Institute，China Medical University. Shenyang 110001,Liaoning, China

Abstract

By means of in situ hybridization and immunohistochemistry, the expression and cellular localization of orphan receptor TR3 and its mRNA were observed. The results showed that both TR3 and its mRNA were expressed in a significant amount in mouse testis, and the expression level of TR3 mRNA was different in current seminiferous tubules. TR3 mRNA was specifically expressed in germ cells, mainly in spermatogonia and less advanced primay spermaaatocytes, whereas TR3 receptor protein was banlylocalized in germ cells. It is suggested that TR3 may play an important role in regulating the early stage of germ cell development in mice.

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引用本文：

穆小民, 刘以训. 孤儿受体TR3在小鼠睾丸中的定位和表达 [J]. 生理学报 1998; 50 (4): .

Cui Hong. . Acta Physiol Sin 1998; 50 (4): (in Chinese with English abstract).