过刊浏览

5-羟色胺和胃动素在狗小肠移行性复合运动调控中的作用

杨春敏, 周吕, 张航, 郝维

中国医学科学院中国协和医科大学基础医学研究所生理研究室；中国医学科学院中国协和医科大学基础医学研究所生理研

摘要

应用慢性植入小肠腔外应力传感器记录清醒狗小肠移行性复合运动（MMC）。在颈外静脉和支配10－15cm肠段的空肠动脉内分别放置一硅胶管以备灌流药物和取血样品用。本研究观察了静脉和动脉注射药物对小肠MMC的影响及MMC不同时相血浆5-羟色胺（5－HT）和胃动素的浓度变化。结果表明：（1）MMC不同时相血浆5-HT和胃动素浓度呈周期性变化，5-HT峰值在胃动素峰值之前出现。血浆5-HT浓度在MMCⅡ相后期达高峰（41．2±1．3）ng／ml，明显高于MMCI相（22．4±1．1）ng／ml（P＜0．01）；而血浆胃动素浓度在MMCⅢ相时达峰值（580.0±30．2）pg／ml，明显高于MMCI相时的水平（140．0±21．0）pg／ml（P＜0．01）。（2）静脉注射胃动素和空肠肠段动脉局部注射5-HT、胃动素分别引起近端小肠和空肠肠段MMCⅢ相收缩。胃动素在空肠肠段引发的收缩振幅高于5－HT。（3）空肠肠段动脉局部灌流5－HT受体拮抗剂、抗胃动素血清、5，6-双羟色胺和河豚毒素（TTX）均可阻断MMCⅢ相在灌流肠段出现和向远端传播。结论：血浆5-HT和胃动素可能是狗小肠MMC的主要启动因素，5-HT释放高峰先于胃动素峰在MMCⅢ相前出现。肠神经系统的5-HT神经元和胃动素神经元在狗小肠MMC调控中起重要作用。

关键词： 移行性复合运动; 5-羟色胺; 胃动素

Cui Hong

First Clinical Institute，China Medical University. Shenyang 110001,Liaoning, China

Abstract

Migrating motor complex (MMC) was recorded by chronically implanted strain gauge transducers on the serosa of small bowel in conscious dogs. Two silastic catheters for injecting ho and taking blood samples were put respectively in a vena jugularis externa and in a jejunal artery which perfused a 10 ~ 15 cm intestinal segment. The present study was undertaken to observe the effect of drugs administered intravenously and intraarterially on MMC and alterations of plasma 5-hydroxytryptamine(5-HT) and motilin in different phases of MMC. The results showed: (1) Levels of plasma 5-HT and motilin showed cyclical fluctuations in different phases of MMC, and 5-HT reached peak before peak of motilin. Plasma 5-HT level reached a peak [(41.2±1 .3) ng/ml] in the later pened of phase Ⅱ, which increased significantly as compared with phase Ⅰ [ (22.4±1.1 )ng/ml (P < 0.01 ), but plasma motilin level had a peak [ (580.0 ± 30.2) pg/ml]in phaseⅢ of MMC, which was higher than that in phase 1 [ (140.0±21 .0) pg/ml](P < 0.01 ).

Key words: ;;

收稿日期：　　录用日期：

通讯作者：　　E-mail:

引用本文：

杨春敏, 周吕, 张航, 郝维. 5-羟色胺和胃动素在狗小肠移行性复合运动调控中的作用[J]. 生理学报 1998; 50 (4): .

Cui Hong. . Acta Physiol Sin 1998; 50 (4): (in Chinese with English abstract).