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降钙素基因相关肽(CGRP)mRNA在大鼠淋巴细胞中的表达

邢立愚, 邢宇彤, 唐跃明, 郭静萱, 王宪

北京医科大学第三医院血管医学研究所

摘要

最近，我们研究发现大鼠胸腺和淋巴结淋巴细胞中存在降钙素基因相关肽（CGRP）样免疫反应活性。应用人工合成的可特异扩增降钙素／降钙素基因相关肽基因部分片断的寡核苷酸引物，通过逆转录-多聚酶链反应（RT－PCR），检测在大鼠脊髓背根神经节、胸腺细胞及肠系膜淋巴结淋巴细胞中是否存在CGRPmRNA，进一步研究大鼠淋巴细胞能否合成CGRP。结果显示，通过RT－PCR从脊髓背根神经节（阳性对照）、胸腺和淋巴结淋巴细胞样品中，都检测到预期的90个碱基对的CGRPCDNA片断。这提示，CGRP除了是一种重要的神经递质外，也可在胸腺细胞和淋巴结淋巴细胞合成。此种CGRP可能与感觉神经源的一样，是免疫和神经系统的共用配基之一。淋巴细胞源的CGRP可能起着免疫细胞调节因子的作用。

关键词： 降钙素基因相关肽; 信使RNA; 逆转录; 多聚酶链反应

Cui Hong

First Clinical Institute，China Medical University. Shenyang 110001,Liaoning, China

Abstract

We recently found that calcitonin gene-related peptide (CGRP)-like immunoreacitivity was present in lymphocytes of fat thymus and lymph node. In older to investigate whether these cells were capable of synthesizing CGRP, CGRP mRNA of rat dorsal root ganglia, thymocytes and mesenteric lymph node lymphocytes were determined by raverse transcription and polymerase chain reaction (RT-PCR) utilizing synthetic oligonucleotides bracketing a portion of the calcitonin/CGRP gene. A discrete band of the expected size of 90 base pairs was found in the dorsal root ganglia (positive control), and in both thymocytes and mesenteric lymph node lymphocytes. These data strongly suggest that CGRP is not only an important neuropeptide, but it is also synthesized in lymphocytes of both thymus and lymph nodes, which is identical to that in sensory neurons. CGRP from lymphocytes may act as an immunomodulator and serve as a common ligand in immune and nervous system.

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引用本文：

邢立愚, 邢宇彤, 唐跃明, 郭静萱, 王宪. 降钙素基因相关肽(CGRP)mRNA在大鼠淋巴细胞中的表达[J]. 生理学报 1998; 50 (4): .

Cui Hong. . Acta Physiol Sin 1998; 50 (4): (in Chinese with English abstract).