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L-苯丙氨酸与血管平滑肌细胞增殖

高平进, 朱鼎良, 詹一鸣, OLIVIERSTEPIEN, PIERREMARCHE, 赵光胜

上海第二医科大学附属瑞金医院. 上海；Pharmacologie CNRSURA1482 France

摘要

本文用氚标胸腺嘧啶核苷掺入DNA合成法测定自发性高血压大鼠（SHR）与正常对照鼠的培养主动脉血管平滑肌细胞（VSMC）增殖，观察L-苯丙氨酸对细胞增殖、细胞生长及原癌基因c-fos、c－myc表达的影响。结果显示：（1）L-苯丙氨酸剂量依赖性地抑制血清、碱性成纤维细胞生长因子及凝血酶诱导的DNA合成；（2）L-苯丙氨酸剂量依赖性地抑制细胞对血清的增殖反应；（3）L-苯丙氨酸抑制血清诱导的c－fos、c－myc的表达；（4）L-酪氨酸，L-组氨酸及D-苯丙氨酸不能产生类似L-苯丙氨酸的抑制作用。这些资料说明L-苯丙氨酸具有直接、特异性的抗VSMC增殖作用，提示L-苯丙氨酸在SHR大鼠中的抗高血压作用可能与血管平滑肌细胞增殖受抑有关。

关键词： L-苯丙氨酸; 血管平滑肌细胞; 细胞增殖

Cui Hong

First Clinical Institute，China Medical University. Shenyang 110001,Liaoning, China

Abstract

Cell growth and proliferation were evaluated in cultured vascular smooth muscle cells (VSMCs) isolated from spontaneously hypertensive rat (SHR) and normotensive Wistar-Kyoto (WKY) rat aortae by measuring [3H]-thymidine incorporation into newly synthesize DNA and by determining cell number, respectively. The results showed that in cultures from both rat strains(1) serum, basic fibroblast growth factor (bFGF)- and thrombin-induced DNA synthesis were inhibited by L-phenylanine dosedependently; (2) L-phenylalanine inhibited cell proliferation in response to serum in a concentration ependent manner; (3) L-phenylalanine inhibited serum-induced proto-oncogene c-fos and c-myc expression;(4)L-tyrosine, L-histidine and D-phenylalanine failed to minic the inhibitory effect of L-phenylalanine. All these data demonstrate that L-phenylalanine could exert a direct and specific antiproliferative effect on VSMCs suggesting that such effect can account for the antihypertensive action of this amino acid observed in SHR.

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引用本文：

高平进, 朱鼎良, 詹一鸣, OLIVIERSTEPIEN, PIERREMARCHE, 赵光胜. L-苯丙氨酸与血管平滑肌细胞增殖[J]. 生理学报 1998; 50 (4): .

Cui Hong. . Acta Physiol Sin 1998; 50 (4): (in Chinese with English abstract).