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红藻氨酸致敏癫痫大鼠海马内AP-1 DNA结合活性和成分的改变

李冬冬, 冯泽辉, 张万琴, 洪昭雄

大连医科大学生理教室

摘要

一次皮下注射惊厥剂量(7.5mg/kg)的红藻氨酸(kainicacid,KA),诱发Fisher344大鼠出现急性癫痫发作,7d后即可形成癫痫敏感大鼠。继用Gelshift、Super-shift和Westernblot方法测定大鼠海马内AP-1DNA结合活性及其组成成分。Gelshift结果显示,癫痫敏感大鼠海马内AP-1DNA结合活性的基础水平较对照组为高;Super-shift实研表明仅Fra和JunD抗体能把AP-1蛋白滞留到较高的位置;Westernblot进一步证明JunD蛋白包括43,39and28kDaJund;又癫痫敏感大鼠若第二次接受癫痫刺激,其AP-1复合物的活性及成分均再次发生改变。以上资料提示AP-1DNA结合活性的增高和高水平的JndD很可能在癫痫敏感性长期持续增高的维持中起重要作用。

关键词: AP-1结合活性; AP-1复合物; 癫痫敏感性; 海马

Cui Hong

First Clinical Institute,China Medical University. Shenyang 110001,Liaoning, China

Abstract

Seizure-sensitive Fisher 344 rats were prepared 7 d after seizure episodes induced by a single subcutaneous administration of convulsive dose (7 .5 mg/kg)of kainic acid. The activator protein-1 (AP1) DNA binding activity and its components in the hippcampus of such rats were examined by Gel shift,Super-shift and Western blot assay.Gel shift assay showed that the basal level of ET-1 im binding activity was significantly higher than that of control rat hippocampus. Super-shift showed that only Fra and Jun D antibodies could super-shift AP-1 complex to a higher position, It suggested that the AP-1 complex was composed of Fra and Jun D in hippocampus of the seizure-sensitive rats. Western blot analyais further confirmed that Jun D proteins contained components of 43,39 and 28 kDa. When secondary seizure stimulations were given to seizure-sensitive rate, the AP-1 DNA binding activity was attenuated and the components of AP-1proteins changed transiently.

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引用本文:

李冬冬, 冯泽辉, 张万琴, 洪昭雄. 红藻氨酸致敏癫痫大鼠海马内AP-1 DNA结合活性和成分的改变[J]. 生理学报 1998; 50 (4): .

Cui Hong. . Acta Physiol Sin 1998; 50 (4): (in Chinese with English abstract).