神经激肽-1(NK-1)受体介导福尔马林诱发的大鼠脊髓c-fos表达
陶元祥, 赵志奇
中国科学院上海脑研究所
摘要
本文用Fos作为背角伤害性反应神经元活动的一个标志物,结合免疫细胞化学和神经药理学方法,观察了速激肽受体拮抗剂对福尔马林诱发的脊髓c-fos原癌基因表达的影响。一侧大鼠后肢跖部皮下注射福尔马林,仅在同侧脊髓背角有c-fos表达。Fos阳性神经元最密集分布于Ⅰ层和Ⅱ层背侧的内侧部,中等量分布于Ⅳ层和Ⅴ层,少量定位于Ⅱ层腹侧部、Ⅲ、Ⅵ和X层。若预先在一侧大鼠后肢跖部皮下注射福尔马林前,鞘内给予神经激肽-1(NK-1)受体拮抗剂L-668,196(0.1,1,10μg)可以产生剂量依赖性福尔马林诱发的背角Fos阳性细胞数量减少(P<0.01),而注射MK-2受体拮抗剂L-659,877(10μg)和NK-3受体拮抗剂SR-142,801(10μg)对福尔马林诱发的背角c-fos表达没有影响(P>0.05)。上述结果表明NK-1受体介导若福尔马林诱发的背角c-fos原癌基因表达,强烈地提示NK-1受体在脊髓伤害性信息传递中起着重要作用。
Cui Hong
First Clinical Institute,China Medical University. Shenyang 110001,Liaoning, China
Abstract
In the present work, the effects of tachykinin receptor antagonists on formalininduced c-fos expression in the rat spinal cord were studied by a combination of immunocytochemical and neuropharmacological methods. Formalin injected into a hindpaw of rat only resulted in c-fos expression in the ipsilateral dorsal horn neurons of the spinal cord. Fos-positive neurons were distributed mainly in medial parts of the lamina Ⅰand the outer lamina Ⅱ. A moderate number of Fos-positive neurons were present in laminae Ⅳ-Ⅴ, and a few in the inner lamina Ⅱand in laminae Ⅲ, Ⅵ and X.Selective NK-1 antagonist L-668, 169 (0.1, 1 and 10μg) administered intrathecally before injection of formalin into a hindpaw significantly reduced the number of Fos-labeled neurons in the dorsal horn in a dose-dependent manner (P < 0.01), while selective NK-2 antagonist L-659, 877 (10μg) and selective NK-3 antagnnist SR-142, 801 (10μg)were ineffective (P> 0.05).
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引用本文:
陶元祥, 赵志奇. 神经激肽-1(NK-1)受体介导福尔马林诱发的大鼠脊髓c-fos表达[J]. 生理学报 1998; 50 (4): .
Cui Hong. . Acta Physiol Sin 1998; 50 (4): (in Chinese with English abstract).