过刊浏览

内皮素-1对肺表面活性物质分泌的调控

罗自强, 孙秀泓

湖南医科大学生理学教研室

摘要

采用离体大鼠非灌流肺，观察生理浓度内皮素-1（ET-1）对肺表面活性物质（PS）分泌的影响。结果表明，10－12和10－10mol／L的ET-1可促进PS磷脂及其主要组分磷脂酰胆碱（PC）的基础分泌；在间歇性肺扩张刺激基础上，ET－1可进一步加强磷脂和PC的分泌。蛋白激酶C（PKC）抑制剂H7可阻断ET-1的促PS分泌效应。培养的肺组织暴露于ET-1（10－12和10－10mol／L）1和4h后，肺组织ET含量显著高于对照组。结果提示，生理水平的ET-1通过PKC的介导参与肺PS分泌的调控；ET-1可诱导肺组织产生ET。

关键词： 内皮素-1; 肺表面活性物质; 蛋白激酶C; 离体非灌流肺

Cui Hong

First Clinical Institute，China Medical University. Shenyang 110001,Liaoning, China

Abstract

The effects of endothelin-1 (ET-1 ) at physiological level on the secretion of pulmonmp surfactant (PS) were studied on isolated nonperfused rat lungs. The results showed that ET-1 (10-12 and 10-10 mol/L) increased the hasal secretion of total phospholipids (TP) and phosphatidylcholine (PC), and potentiated the stimulatory effect of intendttent lung inflation on the secretion of ac and PC. The effect of ET-l could be inhibited by H7, a protein kinase C (PKC) inhihitor. When the cultured lung tissue was exposed respectively to ET-1 (10-12 and 10-10 mol/L) for I h and 4 h,the content of ET in the lung tissue increased significantly. These results suggest that ET-l at the physiological concentration is involved in modulating PS secretion thlough a PKCdependent pathway, and can also promote ET production in lungs.

Key words: ;;;

收稿日期：　　录用日期：

通讯作者：　　E-mail:

引用本文：

罗自强, 孙秀泓. 内皮素-1对肺表面活性物质分泌的调控[J]. 生理学报 1998; 50 (3): .

Cui Hong. . Acta Physiol Sin 1998; 50 (3): (in Chinese with English abstract).