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过量表达Wnt-1基因诱导P19细胞的神经分化

杨靖, 孙红, 边玮, 景乃禾

中国科学院上海生物化学研究所

摘要

Wnt-1基因在小鼠神经发育过程中起着重要作用。该基因在胚胎性癌细胞P19向神经分化过程中存在瞬时性表达。利用克隆到的Wnt－1基因转染P19细胞（Wnt－1／P19），可使细胞不经视黄酸（RA）诱导，自发向神经细胞方向分化。早期神经分化关键基因MASH-1在Wnt－1／P19细胞的神经分化过程中的表达，晚于RA诱导引起的P19细胞的分化过程。

关键词： Wnt-1; 反转录PCR; P19细胞; 过量表达; 神经分化; MASH－1

Cui Hong

First Clinical Institute，China Medical University. Shenyang 110001,Liaoning, China

Abstract

Wnt-1 gene plays important roles during foe development of the mouse central neural system. and shown a transient expression during neuronal differentiation Of P19 EC cells.ac transfected Wnt-1 gene into P19 cells and found the Wnt-1 transfected cells could differentiate into ne~ns without the induction of RA. However, MASH-1, a cmcial gene for the early neural differentiation, was expressed later than P19 cells induced by RA.

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引用本文：

杨靖, 孙红, 边玮, 景乃禾. 过量表达Wnt-1基因诱导P19细胞的神经分化 [J]. 生理学报 1998; 50 (3): .

Cui Hong. . Acta Physiol Sin 1998; 50 (3): (in Chinese with English abstract).