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成年大鼠视皮层(17区)神经元间的染色耦合──离体脑片研究

丁劲, 徐杏珍, 李朝义

中国科学院上海生理研究所神经生物学开放实验室；中国科学院生物物理研究所视觉信息处理开放实验室；中国科学院上海生理研究所

摘要

在离体脑片上，用生物胞素（biocytin）对成年大鼠视皮层神经元进行胞内注射。在标记成功的49例中，21例（42．9％）有染色耦合现象，耦合细胞的个数从2个到5个不等，平均2．8±1．1个。其中，18例为锥体-锥体神经元之间的耦合，2例为锥体-非锥体神经元之间的耦合，1例为非锥体神经元之间的耦合。在耦合细胞中，只有5例的胞体紧靠在一起，其它细胞的胞体之间都有一定的距离，其中有1例达到了635μm。大多数情况下（19／21例），耦合细胞的胞体位于同一层次内，只有2例胞体位于不同层次。在Ⅱ／Ⅲ、Ⅳ和Ⅴ层内都观察到染色耦合神经元，但以Ⅱ／Ⅲ层内发生染色耦合的比率为最高。比较了染色耦合与非耦合神经元的某些电生理特性，没有观察到在它们之间有明显的差别。

关键词： 大鼠; 视皮层; 脑薄片; 染色耦合; 缝隙连接; 生物胞素

Cui Hong

First Clinical Institute，China Medical University. Shenyang 110001,Liaoning, China

Abstract

With intracellular injection of biocytin, forty-nine neurones were successfully labeled in in vitro visual cortex (area 17) slices of the adult rat. Out of the sample dye coupling was found in 21 cases, for each injection the number of the coupled cells varying from 2 to 5 (mean 2.8±1.1). Eighteen couplings were Pyramidal-Pyrdridal, two were Pyrdridal-nonpyrdridal,and one was seen among non-Pyrdridal cells. In most cases,the coupled cells were apart from each other with a certain distance among their cell bodies (mean 43. 1±24 μm). The longest distance was up to 635μm. Most couplings (19/21 ) were observed within the same lamina; ohly two in different laminae. The dye couplings were found in laminaeⅡ/Ⅲ, Ⅳ and V, but mostly in laminae Ⅱ/Ⅲ. We have measured membrane potential and response properties to white matter and intracellular stimulation for the coupled and non-coupled cells; no significant difference was found between them.

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引用本文：

丁劲, 徐杏珍, 李朝义. 成年大鼠视皮层(17区)神经元间的染色耦合──离体脑片研究[J]. 生理学报 1998; 50 (3): .

Cui Hong. . Acta Physiol Sin 1998; 50 (3): (in Chinese with English abstract).