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去甲肾上腺素对大鼠肝细胞延迟外向钾电流的影响

崔桂英, 李金鸣, 刘东举, 崔宏

中国医科大学药理教研室. 辽宁 110001；中国医科大学第一临床学院普外一科. 辽宁 110001

摘要

目前为止国内外尚未见到有关大鼠肝细胞外向钾电流方面的报道。该文用全细胞膜片箝制技术观察了大鼠肝细胞延迟外向钾电流[I_(k)]及去甲肾上腺素等对I_(k)的影响。实验结果表明，在保持电位-50mV、指令电位＋140mV时大鼠肝细胞I_(k)为2．85±1.21nA。去甲肾上腺素明显降低I_(K)，异丙肾上腺素和乙酰胆碱对I_(K)无影响。

关键词： 延迟外向钾电流; 膜片箝制技术; 去甲肾上腺素; 肝细胞

Effects of noradrenaline on delayed outward potassium current in isolated rat hepatocytes

Cui Guiping, La Jinming, Liu Dongju, Cui Hong

Department of Phaamacology, China Medical University. Shenyang 110001,Liaoning, China；First Clinical Institute，China Medical University. Shenyang 110001,Liaoning, China

Abstract

Heretofore no reports are available on the outward potassium currents in rat hepatocytes.Using whole-cell patch clamp tecboque, the effects of noradrenaline and other reagents on the delayed outward potassium current (I_(K)) in isolated rat hepatocytes were investigated. The experimental results showed that the magnitude of I_(K) was 2.85±1.21 nA at a command potential of+140 mV from a holding potential of-50 mV.Noradrenaline decreased I_(K) distincily. Isoprenaline and acetylcholine showed no effect on I_(K) in isolated rat hepatocytes.

Key words: Delayed outward potassium current;Patch clamp method;Noradrenaline;Hepatocyte

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引用本文：

崔桂英, 李金鸣, 刘东举, 崔宏. 去甲肾上腺素对大鼠肝细胞延迟外向钾电流的影响[J]. 生理学报 1998; 50 (2): .

Cui Guiping, La Jinming, Liu Dongju, Cui Hong. Effects of noradrenaline on delayed outward potassium current in isolated rat hepatocytes. Acta Physiol Sin 1998; 50 (2): (in Chinese with English abstract).