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在暴露于蛋白激酶C激动剂的蛙骨骼肌纤维中不存在去横小管

章晓辉, 朱培闳, server.shcnc.ac.cn

中国科学院上海生理研究所. 上海 200031

摘要

过去的工作表明，经12，13-二丁基佛波酯（PDBu，蛋白激酶C激动剂）作用的蛙骨骼肌纤维出现兴奋收缩去耦联。为了了解这种去耦联是否由去横小管引起，本工作研究了细胞内诱发的动作电位。在用渗透压法去横小管后，表明存在完整横小管的动作电位的后去极化逐渐消失。但是，从经1μmol／LPDBu作用12或24h肌纤维中记录到的动作电位依然存在后去极化。上述结果提示，暴露于PDBu的蛙骨骼肌纤维的横小管完整。因而，由PDBu引起的兴奋收缩去耦联的机制仍有待阐明。

关键词： 后去极化; 横小管; 去横小管; 兴奋收编耦联

Absence of detubulation in frog twitch muscle fibres exposed to protein kinase c activator

Zhang Xiaohui, Zhu Peihong

Shanghai Institute of Physiology,Chinese Academy of Sciences. Shanghai 200031,China

Abstract

It has been shown that excitation-contraction(e-c) uncoupling occurred in frog twitch muscle fibres exposed to phorbol 12, 13-dibutyrate (PDBu), a protein dinase C activator. To investigae if this uncoupling results from detubtilation of the transverse tubules, intracellularly evoked action potentials were examined.The after-depolarization of the action potential, representing intact transverse tubules was gradally abolished by osmotic detubulation, while those obtained from the fibres incubated in 1 #mu#mol/L PDBu for 12 or 24 h still possessed the after-depodrization. Thus, it showed that the transverse tubdes were shll intact in frog twitch muscle fibres after exposure to PDBu. The mechedsm of e-c uncoupling induced by PDBu remains to be clarified.

Key words: After-depolarization;Transverse tubule;Detubalation;Excitation-contraction coupling

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引用本文：

章晓辉, 朱培闳, server.shcnc.ac.cn. 在暴露于蛋白激酶C激动剂的蛙骨骼肌纤维中不存在去横小管 [J]. 生理学报 1998; 50 (2): .

Zhang Xiaohui, Zhu Peihong. Absence of detubulation in frog twitch muscle fibres exposed to protein kinase c activator. Acta Physiol Sin 1998; 50 (2): (in Chinese with English abstract).