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精-甘-天冬-丝氨酸四肽对二磷酸腺苷诱导大鼠血小板活化的信号转导的影响

姚兴海, 王培勇, 庞永政, 苏静怡, 唐朝枢

北京医科大学心血管基础研究所. 北京 100083

摘要

该工作在二磷酸腺苷(ADP)活化的大鼠血小板上，观察精-甘-天冬-丝氨酸四肽(RGDS肽)对血小板聚集、蛋白磷酸化、蛋白激酶C和丝裂素活化蛋白激酶活性的影响。结果发现，50μmol／LADP引起血小板聚集时，蛋白激酶C(PKC)及丝裂素活化蛋白激酶(MAPK)活性增加，并引起95和66kD蛋白磷酸化。应用50，100和200μmol／LRGDS肽与其共同孵育，呈浓度依赖地抑制ADP引起的血小板聚集和对PKC及MAP的激活作用，两个作用均呈正相关(r=0.9397和r=0.9368，P＜0.01)。RGDS肽亦呈浓度依赖地抑制ADP诱导的95和66kD蛋白磷酸化，与其抑制PKC及MAPK的激活也明显正相关。上述结果提示，RGDS肽干扰ADP激活血小板的细胞内信号转导可能是其抗栓作用机理之一。

关键词： 精-甘-天冬-丝氨酸四肽(RGDS肽); 血小板聚集; 蛋白磷酸化; 蛋白激酶C; 丝裂素活化蛋白激酶; 信号转导

Effect of Arg-Gly-Asp-Ser(RGDS) peptide on adp-induced signal transduction of activated rat platelet

Yao Ringhai, Wang Peiyong, Pang Yongzheng, Su Jingyi, Tang Chashu

Institute of Cardiovascular Basic Research Beijing Medical University. Beijing 100083,China

Abstract

The purpose of this work was to investigate the effect of Arg-Gly-Asp-Ser (RGDS)peptide on platelet aggregation, protein phosphorylation, protein kinase C (PKC) and thetogen-activated protein kinase (MAPK) activity during platelet achvation. Experiments were performed on ADP activated rat platelets in vivo.Results showed that 50 #mu#mol/LADP, in addition to inducing platelet aggregation, obviously enhanced not only PKC and MAPK activities but also 95 and 66 kD protein phosphorylation. When platelets and ADP were incubated t0gether with 50, 100,200 #mu#mol/L RGDS peptide it was found that the latter markedly inhihited ADP activated platelet aggregation and activation of PKC and MANK, both in a concentration-dependenily manner.RGDS peptide also inhibited 95 and 66 kD protein phosphorylation concentration-dependently and went positively with its activation of PKC and MAPK.

Key words: RGDS peptide;Platelet aggregation;Protein phosphorylation;Protein kinase C; Mitogenactivated protein kinase;Signal transduction

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引用本文：

姚兴海, 王培勇, 庞永政, 苏静怡, 唐朝枢. 精-甘-天冬-丝氨酸四肽对二磷酸腺苷诱导大鼠血小板活化的信号转导的影响 [J]. 生理学报 1998; 50 (2): .

Yao Ringhai, Wang Peiyong, Pang Yongzheng, Su Jingyi, Tang Chashu. Effect of Arg-Gly-Asp-Ser(RGDS) peptide on adp-induced signal transduction of activated rat platelet. Acta Physiol Sin 1998; 50 (2): (in Chinese with English abstract).