过刊浏览

加压素(4-8)对大鼠脑内Ca~(2+)/CaM依赖的蛋白激酶Ⅱ自身磷酸化的影响

乔利亚, 陈秀芳, 顾本贤, 王桐喜, 杜雨苍

中国科学院上海生物化学研究所；中国科学院上海生理研究所

摘要

大鼠皮下注射加压素（AVP）（4-8）1h后，大脑皮层中Ca2+／CaM依赖的蛋白激酶Ⅱ（CaMKⅡ）自身磷酸化程度与对照组比较增高192％，P＜0.001；海马中增高40％，P＜0．05。CaMKⅡ的自身磷酸化程度依赖于Ca2+及CaM的浓度。在用抗CaMKⅡα单克隆抗体对给药1h组样品和对照组样品进行免疫印迹检测时，发现皮下注射AVP（4-8）1h后，大脑皮层中CaMKⅡα亚基的蛋白量没有明显差异。AVP（4－8）的拮抗剂ZDC（C）PR可以明显阻断AVP（4-8）的作用，表明AVP（4-8）刺激CaMKⅡ的活化是通过受体介导的信号转导过程。

关键词： 加压素（4-8）; ZDC（C）PR; 鼠脑 Ca~（2+）／CaM依赖的蛋白激酶Ⅱ; 自身磷酸化; 免疫印迹; 免疫沉淀

MA Yong-Jie

2College of Information Engineering, Taishan Medical University, Taian 271016, China

Abstract

The extent increase of Ca2+/CaM-dependent protein kinase Ⅱ (CaMK Ⅱ)autophosphorylation in various brain regions of rat reached a maximum value, one hour after s. c. drinistration of AVP(4-8). The increase in the cortex amounted to 192% of the control (P< 0.001 ), whi1e in the hippocampus only 40% (P<0.05). The autophosphorylation of CaMKⅡ was dependent on both Ca2+ and CaM. Western blotting with anti-CaMKⅡa monoclonal antibody showed that the content of CaMK Ⅱ α in cortex did not show detectable change in 1 h as compared to the control group.ZDC(C)PR, an antagonist of AVP(4-8), markedly blocked the effect of AVP(4-8), suggesting that AVP (4-8) stimulated CaMK Ⅱautophosphorylation is mediated through its receptor.

Key words: ;;;;;

收稿日期：　　录用日期：

通讯作者：　　E-mail:

引用本文：

乔利亚, 陈秀芳, 顾本贤, 王桐喜, 杜雨苍. 加压素(4-8)对大鼠脑内Ca~(2+)/CaM依赖的蛋白激酶Ⅱ自身磷酸化的影响[J]. 生理学报 1998; 50 (1): .

MA Yong-Jie. . Acta Physiol Sin 1998; 50 (1): (in Chinese with English abstract).