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受体介导内吞对巨噬细胞膜电位、胞浆和溶酶体pH的影响

雷国华, 朴英杰, 吴建春, 鲍永耀, 黄辉, 张薇

第一军医大学中心实验室；第一军医大学南方医院小儿科

摘要

本文利用荧光标记方法测量了刀豆素A、麦芽凝集素、酵母多糖刺激引起的巨噬细胞膜电位、胞浆pH、溶酶体pH的变化。结果显示三种配体均导致细胞膜电位超极化，胞浆pH降低，溶酶体pH升高，三个生理参量趋于稳定时间稍有不同。胞浆pH的降低可能有抑制内吞的作用，溶酶体pH上升是触发溶酶体内容物外排的基本因素。内吞引起的这些变化是细胞代谢过程中自我调节和保护的表现。

关键词： 巨噬细胞; 受体介导内吞; 膜电位; 胞浆pH; 溶酶体pH

MA Yong-Jie

2College of Information Engineering, Taishan Medical University, Taian 271016, China

Abstract

The effects of Con A,WGA,Zymosan A on membrane potential,cytosolic pH and lysosomal pH of ctltured mouse macrophages were measured with fluorescence method.The results showed all the three kinds of ligands caused macrophage membrane potential hyperpolarization,cytosolic acidification and lysosomal alkalization,but reached their stabilized conditions at different times.The acidification of cytosl might inhibit furgher endocytosis.Alkalization of lysosomes was a basic factor for exocytosis of lysosomal contents.These receptor-mediated induced changes reflected cellular self-regulation and self-protection.

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引用本文：

雷国华, 朴英杰, 吴建春, 鲍永耀, 黄辉, 张薇. 受体介导内吞对巨噬细胞膜电位、胞浆和溶酶体pH的影响 [J]. 生理学报 1998; 50 (1): .

MA Yong-Jie. . Acta Physiol Sin 1998; 50 (1): (in Chinese with English abstract).