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电针频率对大鼠脊髓灌流液中SOM和CGRP含量的影响

田津斌, 沈上, 韩济生

北京医科大学神经科学研究中心

摘要

本研究采用放射免疫测定法，分析不同频率电针刺激下大鼠脊髓灌流液中生长抑素（SOM）和降钙素基因相关肽（CGRP）放免活性（ir）的变化。电针频率选择2，15和100H7；分别收集电针前、中、后各30min脊髓灌流液进行测定，实验结果如下：（1）低频（2Hz）电计使脊髓灌流液中SOM－ir水平升高39％（P＜0.05），CGRP－ir降低47％（P＜0.05）；（2）中频电针（15Hz）则相反，使SOM-ri水平下降37％（P＜00.5），CGRP－ir水平增高92％（P＜0．.5）;（3）高频电针（100Hz）使SOM－ir水平减少30％（P＜0．01），但不影响CGRP－ir的水平。上述结果提示：特定频率的外周刺激可选择性地引起脊髓中SOM和CGRP释放的增强或抑制。这一发现可能有一定的临床意义。

关键词： 生长抑素放免活性（SOM－ir）; 降钙素基因相关肽放免活性（CGRP－ir）; 电针; 放射免疫分析; 脊髓; 释放

MA Yong-Jie

2College of Information Engineering, Taishan Medical University, Taian 271016, China

Abstract

Radioimmunoassay(RIA)was used to determine the changes of the immunreactivity(ir)of somatostatin(SOM)and calcitonin gene-related peptide (CGRP)in the spinal perfusate of rat induced by electroacupuncture(EA)of different frequencies.The frequency of EA was chosen to be 2,15 and 100 Hz and the spinal perfusate was collected in three periods of 30 min before,during and after EA.The results indicate:(1)low frequency EA(2Hz)increased the spinal SOM-ir level by 39% (P＜0.05)but decreased that of CGRP-ir by 47%(P＜0.05);(2)conversely,15 Hz EA decreased spinal fluid SOM-ir level by 37%(P＜0.05)but increased tha of CGRP ir by 92%(P＜O.05);(3)100 Hz EA behaved like 15 Hz in inhibiting SOM-ir 1evel (P＜0.01),but without effect on CGRP-ir.The above results show that specific frequency is required for peripheral electrical stimulation to activae or suppress the release of spinal neuropeptides SOM and CGRP,a fact that may have clinical implications.

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引用本文：

田津斌, 沈上, 韩济生. 电针频率对大鼠脊髓灌流液中SOM和CGRP含量的影响 [J]. 生理学报 1998; 50 (1): .

MA Yong-Jie. . Acta Physiol Sin 1998; 50 (1): (in Chinese with English abstract).