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家兔脊髓背半部下行抑制通路中递质作用的补充观察

韩中胜, 赵建础, 乔健天

陕西中医药研究院生理研究室. 陕西, 西安；山西医学院生理教研室. 山西, 太原

摘要

用玻璃微电极记录了麻痹而清醒的家兔束旁核单位对腓总神经强电流刺激发生的痛敏放电,看到此放电可被已在 L_(1)—L_(2)水平切断的脊髓背半部外周端的电流刺激所抑制。在14个可被脊髓刺激明显抑制的痛敏单位中,此脊髓刺激效应在8个单位可因静脉注射酚妥拉明而阻断,其余6个单位则基本不受酚妥拉明注射的影响。进一步观察表明,在8个酚妥拉明有阻断作用的单位,其中7个单位在注射赛庚啶时不表现阻断作用;而在6个酚妥拉明不起阻断作用的单位,注射纳洛酮也不能阻断其脊髓刺激效应。由于作者在过去的工作中已经证明,凡是酚妥拉明有阻断作用的单位注射纳洛酮也都表现出阻断作用,而酚妥拉明无阻断作用的单位对注射赛庚啶却有阻断作用。

关键词： 束旁核; 痛敏放电; 下行抑制; 酚妥拉明; 纳洛酮; 赛庚啶

Supplementary observation on the neurotransmitters involved in the descending inhibitory pathway in the dorsal half of spinal cord in rabbits

Han Zhongsheng, Zhao Jianchu, Qiao Jiantian

Research Department of Physiology, Shanxi Academy of Traditional Chinese Medicine and pharmacy. Xian, Shaanxi, China；Department of Physiology, Shanxi Medical College. Taiyuan, Shanxi, China

Abstract

Our previous experiments showed that nociceptive discharges of parafascicular (Pf) neuron elicited by strong electrical stimulation on peroneal nerve could be markedly inhibited by conditioning stimulation of the caudal stump of dorsal half of spinal cord transected at L_(1)-L_(2) level. We also found that the antinociceptive action of spinal stimulation could be blocked by phentolamine (Phl) in 16 of 27 Pf neurons while it was not effected in 11 remaining neurons by the same agent. In 5 neurons, which had been, influenced by, Phl, naloxone administration could also block the effect of spinal stimulation. In 5 neurons, which had not been influenced by Phl, cyproheptadine administration could block the effect of spinal stimulation in 4. In present experiments we have shown that in 8 neurons influenced by Phl (0.2-0.5 mg/kg, iv.) cyproheptadine administration (1.0-3.0 mg/kg, iv.) can not block the antinociceptive effect of spinal stimulation in 7 and that in 6 neurons, which have not been influenced by Phl, naloxone administration (1.0-1.2 mg/kg, iv.), can not block the effect of spinal stimulation, either. All these results suggest that there may exist at least two groups of fibers descending in the dorsal half of spinal cord to inhibit the transmission. of nociceptive inputs at the spinal level: one is serotonergic and the other may use both noradrenaline and endogenous opioids in series as its neurotransmitters or modulators in the propriospinal circuits.

Key words: Parafascicular neuron;Nociceptive discharges;Descending inhibition;Phentolamine;Nalogone;Cyproheptadine

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引用本文：

韩中胜, 赵建础, 乔健天. 家兔脊髓背半部下行抑制通路中递质作用的补充观察[J]. 生理学报 1985; 37 (1): .

Han Zhongsheng, Zhao Jianchu, Qiao Jiantian. Supplementary observation on the neurotransmitters involved in the descending inhibitory pathway in the dorsal half of spinal cord in rabbits. Acta Physiol Sin 1985; 37 (1): (in Chinese with English abstract).