过刊浏览

辣椒素对离体豚鼠乳头状肌的电生理效应

李茜, 程岩萍, 何瑞荣

河北医科大学基础医学研究所生理室. 石家庄 050017

摘要

应用细胞内微电极技术,观察了辣椒素(capsaicin,CAP)对豚鼠乳头状肌细胞的电生理效应.结果表明:(1)CAP(30、60、120 μmol/L)可浓度依赖地缩短正常乳头状肌的动作电位时程.(2)对部分去极化乳头状肌,CAP(60μmol/L)除缩短动作电位时程外,还使动作电位幅值和超射值降低,零相最大上升速度减慢.(3)预先应用L型钙通道开放剂Bay K8644(0.5 μmol/L),则可阻断CAP(60μmol/L)的电生理效应.(4)预先应用辣椒素受体(vanilloid receptor,VR)阻断剂钌红(20 μmol/L),不影响CAP(60μmol/L)的电生理效应.以上结果提示,CAP能通过非受体途径抑制Ca2+内流,从而影响豚鼠乳头状肌电生理效应。

关键词： 生理学; 辣椒素; 动作电位; 乳头状肌

Electrophysiological effects of capsaicin on guinea pig papillary muscles

Li Qian, Cheng Yanping, He Ruirong

Department of Physiology，Institute of Basic Medicine, Hebei Medical University. Shijiazhuang 050017,China

Abstract

The cardiac electrophysiological effects of capsaicin (CAP) were examined in guinea pig papillary muscles using intracellular microelectrode technique. The results obtained are as follows: (1) the duration of action potential (APD) in normal papillary muscles was decreased by CAP (30, 60, 120 μmol/L) in a concentration-dependent manner; (2) in partially depolarized papillary muscles, 60 μmol/L CAP not only reduced APD, but also decreased the amplitude of action potential,overshoot and maximal velocity of phase 0 depolarization; (3) pretreatment with L-type Ca2 + channel agonist Bay K8644 (0. 5μmol/L) could completely block the effects of CAP (60 μmol/L); (4) pretreatment with ruthenium red (20μmol/L), a vanilloid receptor (VR) blocker, did not affect the actions of capsaicin on papillary muscles. All these results suggest that the effects of CAP on papillary muscles are likely due to a decrease in calcium influx which is not mediated by VR.

Key words: Physiology;Capsaicin;Action potential;Papillary muscles

收稿日期：　　录用日期：

通讯作者：　　E-mail:

引用本文：

李茜, 程岩萍, 何瑞荣. 辣椒素对离体豚鼠乳头状肌的电生理效应[J]. 生理学报 2003; 55 (5): .

Li Qian, Cheng Yanping, He Ruirong. Electrophysiological effects of capsaicin on guinea pig papillary muscles. Acta Physiol Sin 2003; 55 (5): (in Chinese with English abstract).