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磷脂酶A_(2)活力与急性缺氧性肺动脉高压关系的探讨

李惠萍, 何金兰, 全彩娟, 丁劲松

广东医学院呼吸病研究室. 广东, 湛江 524001；湛江师范学院化学系. 广东, 湛江 524068

摘要

通过动物实验探讨磷脂酶A_(2)(PLA_(2))及相关炎性介质在急性缺氧性肺动脉高压形成中的作用。30只SD大鼠随机分为三组：正常对照组、缺氧组及缺氧加地塞米松组。均测定肺动脉压；缺氧30min后，取静脉血和肺组织测定PLA_(2)活力、血小板活化因子(PAF)、前列腺素E_(2)(PGE_(2))、血栓素B_(2)(TXB_(2))。发现缺氧30min后，平均肺动脉压(mPAP)、血及肺中PLA_(2)活力、PGE_(2)、ThB_(2)及PAF均明显增高；用地塞米松预处理则可减轻上述变化。缺氧情况下，PLA_(2)与mPAP，PAF，PGE_(2)和TXB_(2)均呈正相关；PAF，PGE_(2)和TXB_(2)分别又与mPAP呈正相关。提示PLA_(2)通过相关炎性介质在急性缺氧性肺动脉高压的形成中起一定的介导作用。

关键词： 磷脂酶A_(2); 血小板活化因子; 前列腺素; 缺氧性肺动脉高压

The relationship between the activity of phospholipase A_(2) and acute hypoxic pulmonary arterial pressure

Li Huiping, Her Jinlan, Quan Caijuan, Ding Jinsong

Department of Respiratory Internal Medicine, Affiliated Hospital of Guangdong Medical College. Zhangjiang 524001, Guangdong, China

Abstract

The action of phospholipase A_(2) (PLA_(2)) and related inflammatory mediators on the fonnation of hypoxic pulmonary arterial hgnrtension was studied. 30 Sprague-Dawley rats were equally divided into three groups at random: normal control group, hypoic group and the group pretreated with dexamethasone plus hypoxia. The pulmonary arterial pressure (PAP) was measured by inserting a microcatheter into the pulmonary artery.Afer 30 min of hypoxia, the ctivity of PLA_(2), platelet achvating factor (PAF),prostaglandin E_(2) (PGE_(2)) and thromboxane B_(2) (TXB_(2)) were measured in blood and lung tissue, and it was found that the mean pulmonary arterial pressure (mPAP), the PLA2activity, PGE_(2), TXB_(2) and PAF in blood and lung tissue were sighficanily increased; but pretreatment with dexamethasone relieved the changes mentioned above. In hypoxia, a positive correlations was found between the PLA_(2) activity and mPAP, PAF, PGE_(2), TXB_(2) respectively; positive correlations were also found between PAF, PGE_(2), TXB_(2) and mPAP.

Key words: Phospholipase A_(2);Platelet activating factor;Prostaglandin;Hypoxic pulmonary arterial hypertension

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引用本文：

李惠萍, 何金兰, 全彩娟, 丁劲松. 磷脂酶A_(2)活力与急性缺氧性肺动脉高压关系的探讨 [J]. 生理学报 1997; 49 (6): .

Li Huiping, Her Jinlan, Quan Caijuan, Ding Jinsong. The relationship between the activity of phospholipase A_(2) and acute hypoxic pulmonary arterial pressure. Acta Physiol Sin 1997; 49 (6): (in Chinese with English abstract).