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杏仁核内注射CCK-8抑制胃运动的机制

唐明 苏海灵 李春林

青岛大学医学院生理学教研室. 山东, 青岛 266021

摘要

应用胞核团内微量注射和核团电刺激方法，观察杏仁基底内侧核(BMA)对胃运动的影响，分析BMA与下丘脑腹内侧核(VMH)的机能联系。结果如下：(1)双侧BMA内注射八肽胆囊收缩素(CCK-8)(50ng/1μl)，出现胃内压(IGP)和胃运动频率(GMF)显著下降(P＜0.01)。(2)BMA内注射CCK-A受体阻断剂[L364，718](100ng/1μl)或CCK－B受体阻断剂[L365，260](100ng/1μl)，IGP和GMF无明显影响。(3)先给予[L364，718](100ng/1μl)，再给予CCK－8，IGP和GMF的抑制不再出现；而先给[L365，260]，则CCK－8对IGP和GMF的抑制仍出现。(4)在BMA附近如终纹床核杏仁内侧部(BSTIA)和内侧杏仁核(Me)注射CCK-8均未出现IGP和GMP的抑制作用。(5)分别电刺激单侧BMA和VMH，均显著降低IGP和GMF。(6)电损毁单侧VMH后，电刺激同侧BMA或BMA内微量注射CCK-8均不能降低IGP和GMF。这些结果揭示：BMA内CCK－8对IGP和GMF有抑制作用，这种抑制作用是通过BMA内的CCK-A受体激活而实现，CCK-B受体可能不参与。在BMA抑制胃运动的传出通路中,VMH是必须的中介环节。

关键词： 八肽胆囊收缩素; 杏仁基底内侧核; 下丘脑腹内侧核; 胃内压; 胃运动

Inhibition of gastric motility by microinjection of CCK-8 into rat amygdala

Tang Ming

Department of Physiology, Medical College of Qingdao University. Qingdao 260021, Shandong, China

Abstract

Intranulear microinjection and electrical stimulation technique were employed to evaluate the effect of ventromedial hypothalamus (VMH) on the gastric inhibition elicited by basomedial amygdala nucleus (BMA) excitation. The results were as follows: (1)Microinjections of CCk-8 (50 ng/#mu#l) into bilateral BAM resulted in significant decrease in intragastric pressure (IGP) and gastric motility frequency (GMF) (P<0.01). (2)Neither CCK-A receptor antagonis[L364, 718] nor CCK-8 receptor antagonist [L365,260] induced effects on IGP or GMF when given alone. (3) If bilateral BMA were pretreated with [L364, 718], CCK-8 could no longer induce any inhibitory effects, whlie [L365, 260] had no similar suppressive effect. (4) The inhibitory effects were not found in other nuclei in the amygdaloid body, such as bed nucleus of the stria terminaIis intramygdaloid (BSTIA) and amygdaloid nucleus medial (Me). (5) Electrical stimulation of unilatera VMH or BMA would result in the inhibition of IGP and GMF.

Key words: Cholecystokinin-8;Intragastric pressure;Gastric motility;Basomedial amygdaloid;Ventromedial hypothalamus

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引用本文：

唐明 苏海灵 李春林. 杏仁核内注射CCK-8抑制胃运动的机制[J]. 生理学报 1997; 49 (5): .

Tang Ming. Inhibition of gastric motility by microinjection of CCK-8 into rat amygdala. Acta Physiol Sin 1997; 49 (5): (in Chinese with English abstract).