过刊浏览

重组人肝再生增强因子在体外能刺激HTC肝癌细胞DNA合成

杨晓明, 胡志远, 谢玲, 吴祖泽, 贺福初

北京放射医学研究所. 北京 100850

摘要

在成功克隆人肝再生增强因子(hALR)cDNA的基础上，该研究将人ALR编码区cDNA亚克隆于真核瞬间表达质粒pCDNAⅠ，构建了真核表达质粒pCDNAⅠ－hALR，转染cos-7细胞后，表达产物生物活性检测表明：真核表达的hALR在体外能刺激HTC肝癌细胞增殖，这一体外活性的确定为重组人ALR的纯化提供了简洁、可靠的检测方法。

关键词： 肝再生; 人肝再生增强因子; 表达; DNA合成

In vitro stimulation of HTC hepatoma cell growth by recombinant human augmenter of liver regeneration (ALR)

Yang Xiaoming, Hu Zhiyuan, Xie Ling, Wu Zuze, Wu Chutse

Beijing Institute of Radiation Medicine. Beijing 100850, China

Abstract

It was demonstrated that biologically active ALR could be expressed from its cDNA in transient expression experiments in cos-7 cells. The results showed that the cytosolic fraction from the transfected cells with constructed plasmids DNA stimulated of DNA synthesis of in vitro HTC cells in a dose dependent manner. This finding'suggests that the grC hepatoma cell line may be used as a target for bioassay of human ALR in vitro.

Key words: Liver regeneraion;Augmenter of liver regeneration;Expression;DNA synthesis

收稿日期：　　录用日期：

通讯作者：　　E-mail:

引用本文：

杨晓明, 胡志远, 谢玲, 吴祖泽, 贺福初. 重组人肝再生增强因子在体外能刺激HTC肝癌细胞DNA合成[J]. 生理学报 1997; 49 (5): .

Yang Xiaoming, Hu Zhiyuan, Xie Ling, Wu Zuze, Wu Chutse. In vitro stimulation of HTC hepatoma cell growth by recombinant human augmenter of liver regeneration (ALR). Acta Physiol Sin 1997; 49 (5): (in Chinese with English abstract).