过刊浏览

家兔中脑导水管周围灰质中甲七肽样免疫活性物质具有镇痛作用并参与电针镇痛

费宏, 周仲福, 韩济生

北京医学院生理教研室. 北京

摘要

以辐射热照射家兔鼻嘴部皮肤,测定甩头反应潜伏期(ERL)作为痛阈。通过预先埋植的慢性套管向中脑导水管周围灰质(PAG)注射甲七肽降解酶抑制剂 Captopril,观察其镇痛作用以及加强电针镇痛的作用能否被特异的甲七肽抗血清所对抗。(1)单侧 PAG 注射 Captopril240 nmol 的镇痛作用可为同一部位注射甲七肽抗血清(1μl)所翻转,注入甲啡肽抗血清则无效。(2)单侧 PAG 注射 Captopril 60 nmol 有加强电针镇痛的作用。该作用可被1μl 甲七肽抗血清所完全取消,将抗血清量减少到0.1μl 则无效。以上结果说明 PAG 内的甲七肽样免疫活性物质在镇痛和电针镇痛中发挥重要作重。

关键词： 甲七肽; Captopril; 镇痛; 电针镇痛; 中脑导水管周围灰质; 抗血清

Met-enkephalin-arg~(6)-phe~(7) like immunoreactive substances in periaqueductal gray of the rabbit have an analgesic effect and participate in electroacupuncture analgesia

Fei Hung, Zhou Zhongfu, Han Jisheng

Department of Physiology, Beijing Medical College. Beijing, China

Abstract

The present study was performed to evaluate the possible analgesic effect of a newly found C-terminal extended Met-enkephalin (Met-enkephalin-Arg~(6)-Phe~(7), MEAP) in periaqueductal gray (PAG) of the rabbit. Nociception was assessed by measuring the latency of the escape response (ERL) elicited by radiant heat focused on the snout of the rabbit. Captopril, the specific inhibitor of the MEAP degrading enzyme, when injected into PAG through chronically implanted intracranial. cannula at a dose of 240 nmol, produced marked analgesia which could be antagonized by MEAP antiserum (1#mu#l) injected into the same site. Antiserum directed to Met-enkephalin (ME) was not effective at this extent. Microinjection of captopril into PAG at a dose of 60 nmol caused marked potentiation of the effect of electroacupuncture (EA) analgesia. This potentiating effect was completely eliminated by microinjection of 1#mu#l of MEAP antiserum into PAG, being ineffective at a dose of 0.1 #mu#l. These findings suggest that the MEAD-like imrriunoreactive substance in PAG of the rabbit may have an analgesic effect and may play an important role in mediating EA analgesia.

Key words: Met-enkephalin-Arg~(6)-Phe~(7);Captopril;Analgesia;Electroacupuncture analgesia;Periaqueductal gray;Antisera

收稿日期：　　录用日期：

通讯作者：　　E-mail:

引用本文：

费宏, 周仲福, 韩济生. 家兔中脑导水管周围灰质中甲七肽样免疫活性物质具有镇痛作用并参与电针镇痛[J]. 生理学报 1985; 37 (1): .

Fei Hung, Zhou Zhongfu, Han Jisheng. Met-enkephalin-arg~(6)-phe~(7) like immunoreactive substances in periaqueductal gray of the rabbit have an analgesic effect and participate in electroacupuncture analgesia. Acta Physiol Sin 1985; 37 (1): (in Chinese with English abstract).