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小鼠胚胎心肌细胞的分离及电生理特性

骆红艳, 唐明, 胡新武, 宋明柯, 梁华敏, 杜以梅, 张毅

华中科技大学同济医学院生理系. 湖北, 武汉 430030

摘要

本文旨在探索小鼠胚胎心肌细胞的分离方法并观察其电生理特性.应用胶原酶B消化法获得不同时期单个小鼠胚胎心肌细胞;利用全细胞膜片钳技术,记录胚胎心肌细胞的超极化激活的非选择性内向阳离子电流(If)和L-钙电流(ICa-L),并用电流钳记录其自发性动作电位.胚胎心肌细胞通过相差显微镜依据其形态和自发性收缩进行鉴定。

关键词： 胚胎; 心肌细胞; 小鼠; 分离

Isolation and electrophysiological characteristics of embryonic cardiomyocytes in mice

Luo Hongyan, Tang Ming, Hu Xinwu, Song Mingke, Liang Huamin, Du Yimei, Zhang Yi

Department of Physiology, Tongji Medical College of Huazhong University of Science and Technology. Wuhan 430030, China

Abstract

To explore the electrophysiological characteristics of embryonic cardiomyocytes, single embryonic cardiomyocytes were obtained from mice at different periods by a collegenase B digestion approach, whole cell patch clamp recording technique was used to record If and ICa-L, and spontaneous action potential was also recorded. The morphological and spontaneous contractile properties of the isolated cells appeared to be typical embryonic cardiomyocytes when the cells were assessed by phase-contrast microscope. Whole cell recording of isolated cells is easily performed by the whole cell patch clamp technique.

Key words: embryo;Cardiomyocyte;Mice;Isolation

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引用本文：

骆红艳, 唐明, 胡新武, 宋明柯, 梁华敏, 杜以梅, 张毅. 小鼠胚胎心肌细胞的分离及电生理特性[J]. 生理学报 2004; 56 (5): .

Luo Hongyan, Tang Ming, Hu Xinwu, Song Mingke, Liang Huamin, Du Yimei, Zhang Yi. Isolation and electrophysiological characteristics of embryonic cardiomyocytes in mice. Acta Physiol Sin 2004; 56 (5): (in Chinese with English abstract).