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用脑光学成像精确测定猫初级视皮层视野拓扑投射关系

陈昕, 寿天德

复旦大学生命科学学院脑科学研究中心. 上海 200433；中国科学院生物物理研究所视觉信息处理重点实验室. 北京 100101

摘要

利用基于脑内源信号的光学成像和二维互相关分析的方法,对猫初级视皮层17区的视野拓扑离心度(即视网膜-皮层拓扑关系)进行了精确测量.当采用在同一屏幕内处于上下视野的、方位互相垂直的两个相邻光栅刺激时,皮层中一部分区域的绝大部分细胞因同时兴奋而导致方位功能图模糊不清.将这种方位功能图和用单一方位(水平或垂直)全屏光栅刺激所得到的功能图进行比较,通过计算每一像素的互相关系数,从而获得皮层的精确视野拓扑离心度.同时用电生理的方法测量了同一视皮层内的单细胞的感受野位置,证明这种方法得到的视野离心度和光学记录方法得到的相同.因此,本研究为大面积地确定视皮层细胞感受野在视野中的位置提供了一种快速和较准确的方法。

关键词： 视野拓扑离心度; 互相关系数; 17区; 猫; 光学记录

Accurate establishment of the retinotopic topography of area 17 in cats by intrinsic signal optical imaging

Chen Xin, Shou Tiande

Center for Brain Science Research，School of Life Science，Fudan University. Shanghai 200433,China；Laboratory of Visual Information Processing, Institute of Biophyics，Chinese Academy of Sciences.Beijing 100101,China

Abstract

The retinotopic topography of area 17 in cats was measured by optical imaging based on intrin-sic signals. When stimulated with two neighboring gratings oriented orthogonally each other, which were po-sitioned respectively in the upper and lower visual fields, one piece of cortex that had the retinal projectioncorresponding to the area around the border of the two stimulus gratings became blurred in the resultantfunction orientation map, because the neurons in this site received excitatory signals from both the horizontaland the vertical gratings via indirect ways. This functional map of the same cortex was compared with that e-licited only by a horizontal or vertical grating stimulation in the whole visual field. Accordingly , the accu-rate position of the retinotopic eccentricity of the cortex in visual field can be demarcated by calculating thecross correlation coefficient of the two functional maps. Furthermore, compared with the electrophysiologicalmeasure of receptive fields of single cortical neurons,the retinotopic eccentricities revealed by optical imaging were identical. This experiment provides a fast and relatively accurate method to calculate the retinotopic eccentricities in a large cortical area of the visual cortex.

Key words: Retinotopic eccentricity;Cross correlation coefficient;Area 17;Cat;Optical imaging

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引用本文：

陈昕, 寿天德. 用脑光学成像精确测定猫初级视皮层视野拓扑投射关系[J]. 生理学报 2003; 55 (5): .

Chen Xin, Shou Tiande. Accurate establishment of the retinotopic topography of area 17 in cats by intrinsic signal optical imaging. Acta Physiol Sin 2003; 55 (5): (in Chinese with English abstract).