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电针镇痛时大鼠外侧网状旁巨细胞核中内阿片肽的变化

周丽, 姜建伟, 吴根诚, 曹小定

上海医科大学神经生物学教研室. 上海 200032

摘要

该文应用放射免疫测定的方法观察了大鼠外侧网状旁巨细胞核(RPGL)推挽灌流液中内阿片肽含量在针刺前后的变化。电针组动物经电针20min后,RPGL灌流液中亮氨酸脑啡肽(LEK)的含量显著高于对照组(P<0.05)。高针效组动物电针后RPGL灌流液中β-内啡肽(β-EP)的含量显著高于对照组(P<0.05),而低针效组动物电针后β-EP的含量有减少趋势。动物电针后痛阈的升高与RPGL中LEK及β-EP的含量增多呈正相关(P<0.05);电针20min后动物RPGL灌流液中强啡肽A_(1-13)[DynA(1-13)]的含量与对照组比较仅显示一微小的增加(P>0.05)。

关键词： 亮氨酸脑啡肽; β-内啡肽; 强啡肽A_(1-13); 电针镇痛; 推挽灌流; 外侧网状旁巨细胞核

Changes of endogenous opioid peptides content in rpgl during acupuncture analgesia

Zhou Li, Jiang Jianwei, Wu Gencheng, Cao Xiaoding

Department of Neurobiology, Shanghai Medical University. Shanghai 200032, China

Abstract

By push-pull perfusion technique and RIA of neuro-peptides，it was found that
after 20 minutes' electroacupunture (EA)，the release of LEK from the RPGL in the EA group was significantly higher than that in the control group (P<0.05). The release of #beta#-EP from the RPGL in the EA group with higher efficacy was significantly higher than that in the control group (P<0.05),but no significant changes were found in the EA group with lower efficacy. There was a positive correlation between the changes of release of LEK or #beta#-EP and the increase of pain threshold.The release of DynA_(1-13) tends to show a barely significant extent(P>0.05).The results indicate that acupuncture analgesia is able to increase the release of LEK and #beta#-EP in the RPGL.

Key words: Leu-enkephalin;#beta#-endorphin;Dynorphin A_(1-13);Electroacupuncture analgesia;Push-pull perfusion;Nucleus reticularis paragigantocellularis lateralis

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引用本文：

周丽, 姜建伟, 吴根诚, 曹小定. 电针镇痛时大鼠外侧网状旁巨细胞核中内阿片肽的变化[J]. 生理学报 1993; 45 (1): .

Zhou Li, Jiang Jianwei, Wu Gencheng, Cao Xiaoding. Changes of endogenous opioid peptides content in rpgl during acupuncture analgesia. Acta Physiol Sin 1993; 45 (1): (in Chinese with English abstract).