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瘦素对GH3细胞分泌和凋亡的影响

刘亚莉, 钟延清, 迟素敏, 朱运龙

第四军医大学基础部生理教研室.陕西,西安 710032

摘要

该文旨在探讨瘦素(leptin)对垂体瘤GH3细胞的生长激素(growth hormone,GH)分泌的作用及可能机制。作者观察了leptin对GH3细胞生长激素的分泌、细胞的增殖和凋亡的影响,结果显示:leptin(1、10和100 nmol/L)对GH3细胞的基础GH分泌有抑制作用({sl P}<0.05),并存在剂量依赖效应。用10 nmol/L的leptin作用30 min、1和3 h对GH分泌无明显影响,而作用1、2和3 d则可抑制GH分泌({sl P}<0.05)。应用噻唑蓝(MTT)比色分析法和流式细胞仪研究leptin对GH3细胞增殖和凋亡的影响,作者发现leptin对GH3细胞的增殖有抑制作用,并存在剂量依赖效应;同时leptin可减低GH3细胞的S期细胞比例,而G_(1)期的细胞比例明显增加,进入2相和4相的凋亡细胞比例增加。上述结果表明,leptin可抑制GH3细胞的基础GH分泌,其作用可能是通过抑制GH3细胞的DNA合成,促进GH3细胞的凋亡,从而影响GH的分泌。

关键词： 瘦素; 生长激素; GH3细胞; 凋亡

Effect of leptin on growth hormone secretion and apoptosis of GH3 cells

Liu Yali, Zhong Yanqing, Chi Sumin, Zhu Yunlong

Department of Physiology, the Fourth Military Medical University.Xi'an 710032,Shaanxi

Abstract

In order to investigate the effect of leptin on the secretion of rat pituitary adenoma GH3 cell and its mechanisms, we observed the effect of leptin on the growth hormone secretion, GH cells proliferation and apoptosis of GH3 cells. The results indicated that leptin at 1nmol/L--100nmol/L could inhibit the basal growth hormone secretion of GH3 cells in a dose dependent manner (P＜0.05). Short-term treatment of leptin (10nmol/L) for 30 min, 1h and 3h did not affect basal GH secretion. However,treatment of the GH3 cells with leptin (10nmol/L) for 1d or longer resulted in an inhibition of GH secretion. We also found that leptin reduced the proportion of cells in S phase and increased the proportion of cells in G1 phase. We used flow cytometery (FCM) to study the effect of leptin on the GH3 apoptosis.The result indicated that leptin increased the proportion of GH3 cells in 2 and 4 phase. These results demonstrate that leptin inhibits the basal GH secretion of GH3 cells, which may be due to the inhibition of DNA synthesis and advanced apoptosis of GH3 cells.

Key words: leptin;growth hormone;GH3 cell;Apoptosis

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引用本文：

刘亚莉, 钟延清, 迟素敏, 朱运龙. 瘦素对GH3细胞分泌和凋亡的影响[J]. 生理学报 2005; 57 (2): .

Liu Yali, Zhong Yanqing, Chi Sumin, Zhu Yunlong. Effect of leptin on growth hormone secretion and apoptosis of GH3 cells. Acta Physiol Sin 2005; 57 (2): (in Chinese with English abstract).