过刊浏览

鞘内注射#gamma#--氨基丁酸转运体抑制剂NO--711抑制坐骨神经慢性挤压伤大鼠神经病理性痛觉过敏

朱珊珊, 曾因明, 王俊科, 严蓉, 聂鑫, 曹君利

中国医科大学第一临床学院.辽宁,沈阳 110001；江苏省麻醉医学研究所,徐州医学院江苏省麻醉学重点实验室.江苏,徐州 221002

摘要

为研究#gamma#--氨基丁酸转运体在神经病理性痛中的作用,实验用坐骨神经慢性挤压伤致神经病理性痛模型大鼠,以清醒大鼠分别对辐射热刺激和机械性触觉刺激的缩腿潜伏期和机械阈值为指标,分为NS组、N5组、N10组、N20组、N40组5组,分别在坐骨神经结扎前和结扎后第三天鞘内给予生理盐水或不同剂量的#gamma#--氨基丁酸转运体特异性抑制剂NO--711(5、10、20、40#mu#g),观察鞘内注射NO--711对大鼠热痛敏和触诱发痛的影响。结果表明,NO--711可显著抑制神经病理性痛大鼠的热痛觉过敏和触诱发痛({sl P}<0.05,{sl P}<0.01),其抑制作用持续时间最长分别可达2 h(N40组)和4 h(N20组),其抗热痛敏作用呈剂量依赖性。坐骨神经结扎前鞘内给予不同剂量的NO--711可不同程度地延迟坐骨神经结扎所致的热痛觉过敏的发生,但不能延迟结扎所致的触诱发痛的发生。结果表明#gamma#--氨基丁酸转运体抑制剂在神经病理性痛大鼠具有抗热痛敏和抗触诱发痛的作用。

关键词： 神经病理性痛; #gamma#-氨基丁酸转运体; 热痛觉过敏; 鞘内注射

Inhibition of thermal hyperalgesia and tactile allodynia by intrathecal administration of#gamma#--aminobutyric acid transporter--1 inhibitor NO--711 in rats with chronic constriction injury

Zhu Shanshan, Zeng Yinming, Wang Junke, Yan Rong, Nie Xin, Cao Junli

Department of Anesthesiology,the First Affiliated Hospital,China Medical University.Shenyang 110001,Liaoning；China

Abstract

The present study was undertaken to explore the role of #gamma#-aminobutyric acid transporters in the neuropathic pain. On the chronic constriction injury (CCI) rats 4 doses (5, 10, 20, 40 #mu#g in group N5, N10, N20, N40, respectively) of specific #gamma#-aminobutyric acid transporter-1 inhibitor NO-711 or normal saline (in group NS) were intrathecally administered before sciatic nerve ligation (pretreatment) or at the third day after ligation (post-treatment). The paw withdrawl latency (PWL) from a noxious thermal stimulus and paw withdrawl mechanical threshold (PWMT) of von Frey filament was used as measure of thermal hyperalgesia and tactile allodynia respectively. The results demonstrated that post-treatment of NO-711 significantly suppressed thermal hyperalgesia and allodynia in CCI rats (P<0.05, P<0.01), the inhibitory effect lasted for 2 h (N40 group) and 4 h (N20 group) respectively. NO-711 inhibited thermal hyperalgesia induced by CCI in a dose-dependent manner. Intrathecal pretreatment with different doses of NO-711 delayed the occurrence of thermal hyperalgesia, but could not delay the emergence of allodynia induced by CCI. This study indicates that #gamma#aminobutyric acid transporter inhibiwr has anti-thermal hyperalgesia and anti-tactile allodynia effects in neuropathic rats.

Key words: neuropathy;amino acid transport systems;thermal hyperalgesia;injections, spinal

收稿日期：　　录用日期：

通讯作者：　　E-mail:

引用本文：

朱珊珊, 曾因明, 王俊科, 严蓉, 聂鑫, 曹君利. 鞘内注射#gamma#--氨基丁酸转运体抑制剂NO--711抑制坐骨神经慢性挤压伤大鼠神经病理性痛觉过敏[J]. 生理学报 2005; 57 (2): .

Zhu Shanshan, Zeng Yinming, Wang Junke, Yan Rong, Nie Xin, Cao Junli. Inhibition of thermal hyperalgesia and tactile allodynia by intrathecal administration of#gamma#--aminobutyric acid transporter--1 inhibitor NO--711 in rats with chronic constriction injury. Acta Physiol Sin 2005; 57 (2): (in Chinese with English abstract).