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实验性变态反应性脑脊髓炎大鼠发病中脑组织血红素氧合酶-1基因和蛋白表达的动态变化

檀国军, 朱一飞, 曹翠芳, 赵晓云, 马常升, 杨天祝

河北医科大学第二医院神经内科. 河北, 石家庄 050000；河北省人民医院. 河北

摘要

为探讨脑组织血红素氧合酶-1(heme oxygenase-1,HO-1)对实验性变态反应性脑脊髓炎(experimental allergic encephalomyelitis,EAE)的作用,分别应用逆转录-聚合酶链反应(RT-PCR)和免疫组化技术测定了豚鼠脊髓生理盐水匀浆+完全福氏佐剂诱导EAE大鼠1、7、14、21 d时,脑组织HO-1基因和蛋白表达的动态变化,并观察与症状之间的关系。

关键词： 神经生物学; 脑脊髓炎; 逆转录聚合酶链反应; 血红素氧合酶; 穹窿下器

Dynamic changes of heme oxygenase-1 protein and mRNA in the brains of rats with experimental allergic encephalomyelitis

Tan Guojun, Zhu Yifei, Cao Cuifang, Zhao Xiaoyun, Ma Changsheng, Yang Tianzhu

Department of Neurology, the 2nd Hospital of Hebei Medical Univeristy. Shijiazhuang 050000, China；People's Hospital of Hebei Province. Shijiazhuang 050000, China；Department of Neurobiology, Hebei Medical University. . Shijiazhuang 050000, China

Abstract

In order to ivestigate the role of heme oxygenase-1 (HO-1) in the molecular mechanism of experimental allergic encephalomyelitis (EAE), Which was induced by guinea pig spinal cord homogenate+complete freund adjuvant on Wistar rats, We observed the gene of HO-1 and its protein expression with reverse transcriptase polymerase chain reaction (RT-PCR) and immunohistochemistry 1,7,14 and 21 d after EAE induction in rats.

Key words: neurobiology;Encephalomyelitis;Reverse transcriptase polymerase chain reaction;Heme oxygenase;Subfornicla organ

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引用本文：

檀国军, 朱一飞, 曹翠芳, 赵晓云, 马常升, 杨天祝. 实验性变态反应性脑脊髓炎大鼠发病中脑组织血红素氧合酶-1基因和蛋白表达的动态变化[J]. 生理学报 2004; 56 (5): .

Tan Guojun, Zhu Yifei, Cao Cuifang, Zhao Xiaoyun, Ma Changsheng, Yang Tianzhu. Dynamic changes of heme oxygenase-1 protein and mRNA in the brains of rats with experimental allergic encephalomyelitis. Acta Physiol Sin 2004; 56 (5): (in Chinese with English abstract).