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慢性吸烟大鼠气道平滑肌大电导的钙激活钾通道和Kv1.5表达的变化

叶红, 马万里, 杨木兰, 刘声远, 王迪浔

华中科技大学同济医学院病理生理系, 卫生部呼吸系疾病重点实验室；华中科技大学同济医学院附属协和医院呼吸内科

摘要

复制大鼠的慢性吸烟模型,采用气道反应性的测定、HE染色、免疫组织化学染色、原位杂交和免疫印迹实验等方法,观察吸烟对大鼠支气管平滑肌大电导的钙激活的钾通道(BKca)和电压依赖性延迟整流钾通道Kv1.5蛋白和mRNA表达的影响,以阐明吸烟引起的气道高反应性发病机制中钾通道表达变化的作用.结果显示:(1)慢性吸烟可降低大鼠大气道和小气道BKca和Kv1.5蛋白和mRNA表达。

关键词： 吸烟; 支气管平滑肌; 钾通道

Effect of chronic cigarette smoking on large-conductance calcium-activated potassium channel and Kv1.5 expression in bronchial smooth muscle cells of rats

Ye Hong, Ma Wanli, Yang Mulan, Liu Shengyuan, Wang Dixun

Department of Pathophysiology pathology, Tongji Medical College, HuaZhong University of Science and Technology, Pulmonary Disease Laboratory, Ministry of Health of China. Wuhan 430030, China；Department of Respiratory medicine, Xiehe hospital, Tongji Medical College, HuaZhong University of Science and Technology. Wuhan 430030, China

Abstract

To investigate the role of potassium channels in the pathogenesis of airway hyperresponsiveness induced by cigarette smoking, the alteration in expression of large-conductance calcium-activated potassium channel (BKca) and voltage-dependent delayed rectifier potassium channel (Kv1.5) in bronchial smooth muscle cells were investigated in chronic cigarette smoking rats. Airway responsiveness was determined, hematoxylin and eosin staining, immuno-histochemistry, in-situ hybridization and western blottechniques were used. The results showed: (1) Chronic cigarette smoking down-regulated the protein synthesis and mRNA expression of BKca and Kv1.5 in bronchial and bronchiolar smooth muscles.

Key words: Cigarette smoking;Bronchial smooth muscle cells;Potassium channel

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引用本文：

叶红, 马万里, 杨木兰, 刘声远, 王迪浔. 慢性吸烟大鼠气道平滑肌大电导的钙激活钾通道和Kv1.5表达的变化[J]. 生理学报 2004; 56 (5): .

Ye Hong, Ma Wanli, Yang Mulan, Liu Shengyuan, Wang Dixun. Effect of chronic cigarette smoking on large-conductance calcium-activated potassium channel and Kv1.5 expression in bronchial smooth muscle cells of rats. Acta Physiol Sin 2004; 56 (5): (in Chinese with English abstract).