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自发性高血压大鼠和Wstar Kyoto大鼠心血管组织肾上腺髓质素和肾上腺髓质素原N末端20肽的水平

齐永芬, 卜定方, 石彦荣, 李菊香, 庞永正, 唐朝枢

北京大学第一医院心血管病研究所，北京100034 ；北京大学医学部生理学系，北京100083

摘要

工作观察了自发性高血压大鼠(SHRd)和Wistar Kyoto ( WK))大鼠心肌和主动脉肾上腺髓质素(a-drenomedullin, AD哟和肾上腺髓质素原N末端20肤( proadrenomedullin N terminal 20 peptide , PAMP)的水平。以放射免疫分析方法测定血浆、心肌和主动脉ADM含量。用竞争性定量逆转录多聚酶链式反应(RT PC助方法测定心肌和主动脉ProADM m RNA含量。结果发现，SHRs心肌和主动脉ProADM m RNA水平分别比WKY大鼠高66.7%和73 %(均P<
0.01)。SHRs血浆心肌和主动脉ADM i:含量分别较WKY大鼠高29%,76.7%和79均P<0.01)。SHRs血浆心肌和主动脉PAMP- i:水平分别较WK、大鼠高42.5%(P<0.01)}47.2%(P<0.01)和27.3%(P<0.05)。另外，SHRs的ADM和PAMP的比值较WKY大鼠明显增高(心肌和主动脉分别为2.。士。.25二1.64士。.3和2.2士。.18二1.56士。.28)。结果提示，SHRs心肌和主动脉ProADM基因表达上调，ADM和PAMP水平升高，但二者升高的比例不一致。SHRs的ADM和
PAMP升高不一致的病理生理意义有待进一步研究。

关键词： 病理生理学; 肾上腺髓质素; 肾上腺髓质素原N末端20肽; 自发性高血压大鼠; m RNA

Levels of adrero medullin and proadrero medullin N-terminal 20 peptide in myocardium and aorta of spontaneously hypertersive ratsand Wstar- Kyoto rats

QI Yong Fen, Bu Dingfang, Shi Yanrong, Li Juxiang, Pang Yongzheng, Tang Chaoshu

Institute of Cardiovascular Disease，Peking University First Hospital，Beijing 100034；Department of Physiology，Peking University Health Science Center, Beijing 100083

Abstract

In this study，observed the levels of adrenomedullin(AD哟and proadrenomedullin Nterminal 20 peptide(PAMP) in myocardium and aorta of spontaneously hypertensive rats(SHRs) in comparison with Wistarkyoto(WKY) rats .Contents of ADM and PAMP were measured by radioimmunoassay(RIA) in plasma，myocardium and aorta .The amount of Pro' ADM mRNA of myocardium
and aorta was determined by competitive quantitative reverse transcription polymerase chain reaction(RT PCR).In SHRs the amounts of Pro, ADM mRNA of myocardium and aorta were 66.7%(P(0.01)and 73%(P(0.01)higher than those in WKY rat，respectively .In SHRs，the levels of ADM in plasma，myocardium and aorta were 29%，76.7%and 79%(all P(0.01)higher than those in WKY rats，ectively .The level of PAMP in SHRs was increased by 42.5%in plasma(P(0.01)，47.2%in myocardium(P(0.0.1)and 27.3%in aorta(P(0. 05) compared to WKY rats，tively .In addition，the ratio of ADM content to PAMP content in SHRs group was increased compared with that in WKY group(2.0士0.25ts1.64士0. 3 and 2. 2士0.18ts1.56士0. 28，in myocardium and aorta，respectively，P(0.01).These results suggest that ProADM gene expression is up- regulated and the increase in ADM and PAMP is different in SHRs .The significance of inconsistency of increase in ADM and PAMP in SHRs needs to be further investigated.

Key words: pathophysiology;Adrenomedullin;proadrenomedullin N terminal 20 peptide(PAMP);Spontaneously hypertensive rat;m RNA

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引用本文：

齐永芬, 卜定方, 石彦荣, 李菊香, 庞永正, 唐朝枢. 自发性高血压大鼠和Wstar Kyoto大鼠心血管组织肾上腺髓质素和肾上腺髓质素原N末端20肽的水平[J]. 生理学报 2003; 55 (3): .

QI Yong Fen, Bu Dingfang, Shi Yanrong, Li Juxiang, Pang Yongzheng, Tang Chaoshu. Levels of adrero medullin and proadrero medullin N-terminal 20 peptide in myocardium and aorta of spontaneously hypertersive ratsand Wstar- Kyoto rats . Acta Physiol Sin 2003; 55 (3): (in Chinese with English abstract).