过刊浏览

5,7-双羟色胺损毁大鼠中缝背核后底丘脑核的神经活动增强

刘健, 褚玉霞, 冯洁, 王勇, 张巧俊, 许临平

西安交通大学医学院生理学教研室.陕西，西安 710061；西安交通大学第二医院神经内科.陕西，西安 710004

摘要

采用玻璃微电极在体细胞外记录法，观察了5,7-双羟色胺（5,7-dihydroxytryptamine, 5,7-DHT）损毁大鼠中缝背核(dorsal raphe nucleus, DRN)后，底丘脑核（subthalamic nucleus, STN）神经元电活动的变化。结果发现，对照组和DRN损毁组大鼠STN神经元的放电频率分别是6.93±6.55 Hz和11.27±9.31 Hz，DRN损毁组大鼠的放电频率显著高于对照组（P<0.01）。在对照组大鼠，13%的神经元呈现规则放电，46％有不规则放电，41%有爆发式放电；而在DRN损毁组大鼠，具有规则、不规则和爆发式放电的神经元比例分别为9%、14%和77%，爆发式放电的STN神经元比例明显高于对照组（P<0.01）。结果显示，DRN损毁后大鼠STN神经元的放电频率增高，爆发式放电增多，提示在正常大鼠DRN抑制STN神经元的活动。

关键词： 底丘脑核; 中缝背核; 5,7-双羟色胺; 大鼠

Increased subthalamic neuronal activity after dorsal raphe nucleus lesion by 5,7-dihydroxytryptamine in the rat

Liu Jian, Zhu Yuxia, Feng Jie, Wang Yong, Zhang Qiaojun, Xu Linping

Department of Physiology, School of Medicine, Xi'an Jiaotong University.Xi’an 710061,Shaanxi；China

Abstract

In vivo extracellular recordings were made in the subthalamic nucleus (STN) of intact control rats and rats with 5,7-dihydroxytryptamine (5,7-DHT) –produced lesion of dorsal raphe nucleus (DRN). The results showed that the firing rate of STN neurons in control rats and DRN-lesioned rats were 6.93±6.55 Hz and 11.27±9.31 Hz, respectively, the firing rate of DRN-lesioned rats was significantly increased when compared to control rats (P<0.01). In control rats, 13% of STN neurons discharged regularly, 46% irregularly and 41% in bursts. In DRN-lesioned rats, 9% of STN neurons discharged regularly, 14% irregularly and 77% in bursts, the percentage of STN neurons firing in bursts was obviously higher than that of control rats (P<0.01). In addition, the mean interspike interval coefficient of variation of STN neurons in control rats and DRN-lesioned rats were 0.05±0.04 and 0.11±0.09, respectively, the mean interspike interval coefficient of variation of DRN-lesioned rats being significantly higher than that of control rats (P<0.001). These results show that the firing rate and the bursting pattern rate of neurons in STN of DRN-lesioned rats are increased significantly, suggesting that DRN inhibits the neuronal activity of the subthalamic neurons in the intact rat.

Key words: Subthalamic nucleus;Dorsal raphe nucleus;5,7-dihydroxytryptamine;Rat

收稿日期：　　录用日期：

通讯作者：　　E-mail:

引用本文：

刘健, 褚玉霞, 冯洁, 王勇, 张巧俊, 许临平. 5,7-双羟色胺损毁大鼠中缝背核后底丘脑核的神经活动增强[J]. 生理学报 2005; 57 (1): .

Liu Jian, Zhu Yuxia, Feng Jie, Wang Yong, Zhang Qiaojun, Xu Linping. Increased subthalamic neuronal activity after dorsal raphe nucleus lesion by 5,7-dihydroxytryptamine in the rat. Acta Physiol Sin 2005; 57 (1): (in Chinese with English abstract).