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微丝在低渗牵张诱导毒#艹四早#碱电流增加中的作用

王佐好, 禹永春, 崔艺峰, 李林, 郭慧淑, 李在琉, 许文燮

延边大学医学院1消化生理研究室,附属医院中心实验室，延吉133000

摘要

在急性分离的豚鼠胃窦平滑肌细胞上，利用膜片钳技术的传统全细胞模式记录离子电流的方法，探讨微丝在低渗牵张诱导毒覃碱电流增加中的作用。当豚鼠胃窦平滑肌细胞的膜电位钳制在一20 m V时，灌流液中50umol/ L卡巴胆碱(carbachol , CCh)或电极内液中0.5 mmol/L GTPYS均可引导毒覃碱电流(muscarinic current lCce) ,2 m Os mol/ L)分别使其增加145士27%和183士30;当电极内液中加入20 umol/ L的细胞松弛B(一种微丝骨架的解聚剂)时，低渗牵张使1 cce只增加70士6;而电极内液中加入20 umol/ L的鬼笔环肤(一种微丝骨架的稳定剂)则使Icce增加了545士81。结果表明，低渗牵张可增加由卡巴胆碱或GTPYS诱导的毒覃碱电流，微丝参与调节低渗牵张诱导豚鼠胃窦平滑肌细胞Icce增加的作用。

关键词： 毒#艹四早#碱电流; 胃窦平滑肌; 牵张; 微丝

Role of摄滋 actin nncrolilamert in hypos nntic membrane stretch induced increase in mucarirac current of少。内gastric my

WANG Zuo Yu, YU Yongr Chun, CUI Yi- Feng, LI Lin, GUO Hui-Shu, LI Zai Liu, Xu Wen Xie，

Research Laboratory of Digestive Physiology and Research Center of Affiliated Hospital，Yanbian University College of Medicine，Yanji 133000

Abstract

To investigate the relationship between cytoskeleton and hypos motic membrane stretch-induced increase in muscarinic current，the role of actin microfilament in hyposmotic membrane stretc卜induced increase in muscarinic current was studied with the whole-cell patch clamp technique in guinea-pig gastric myocytes .In this study，the muscarinic current was induced by carbachol(50 umol/ L) or GTPYB(0.5 mmol/L).The results showed that hyposmotic superfusate(202mOsmol/ L) increased carbachol-induced current(lCCs) by 145士27%and increased GTPYS-induced current by 183士30%;but in the presence of cytochalasirr B(cyt- B，20 umol/ L)，an actin cytoskeleton disrupter，posmotic membrane stretch increased 1CC6 by 70士6%.However，hypos motic membrane tretch induced increase in 1CC6 was potentiated to 545士81% by phalloidin(20 umol/ L)，an actin microfilament stabilizer.The results demonstrated that hyposmotic membrane stretch increased the muscarinic currents induced by carbachol or GTPYS and that the actin microfilament is involved in the process n guinea7pig gastric myocytes.

Key words: muscarinic currents;gastric myocytes;stretch;actin microfil ament

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引用本文：

王佐好, 禹永春, 崔艺峰, 李林, 郭慧淑, 李在琉, 许文燮. 微丝在低渗牵张诱导毒#艹四早#碱电流增加中的作用[J]. 生理学报 2003; 55 (2): .

WANG Zuo Yu, YU Yongr Chun, CUI Yi- Feng, LI Lin, GUO Hui-Shu, LI Zai Liu, Xu Wen Xie，. Role of摄滋 actin nncrolilamert in hypos nntic membrane stretch induced increase in mucarirac current of少。内gastric my. Acta Physiol Sin 2003; 55 (2): (in Chinese with English abstract).