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大鼠颈部淋巴引流阻断后海马bcl-2、bax表达和细胞凋亡的动态变化

李育娴, 夏作理, 陈连璧, 叶文静, 杨明峰, 孙巧玲

山东大学医学院生理研究所.山东，济南 250012；泰山医学院脑微循环研究所.山东，泰安 271000；泰山医学院生物技术制药教研室.山东，泰安 271000

摘要

为了研究淋巴滞留性脑病中海马bcl-2、bax基因表达和细胞凋亡的动态变化，该实验用阻断大鼠颈部淋巴引流的方法制备淋巴滞留性脑病模型，术后1、2、3、5、7和14天处死动物，H＆E染色观察海马组织结构变化，TUNEL荧光标记检测原位细胞凋亡，RT-PCR检测海马bcl-2和bax的mRNA表达。结果显示脑组织有水肿的结构变化, 第5天最明显。海马TUNEL阳性细胞数从术后2天开始增多，5天达最高值。bax表达于术后1天开始增高，第2天即达最高值。bcl-2表达于术后1天开始降低，5天达最低值。第14天上述指标均恢复到对照组水平。研究表明，阻断颈部淋巴引流所导致的淋巴滞留性脑病中海马bcl-2和bax的表达发生变化，而且海马神经细胞的死亡以凋亡为主。

关键词： 大鼠，Wistar; 淋巴结切除; 脑损伤; 细胞凋亡; 基因; bcl-2

The changes of bcl-2、bax expression and neuron apoptosis in the hippocampus after the blockade of cervical lymphatics of rats

Li Yuxian, Xian Zuoli, Chen Lianbi, Ye Wenjing, Yang Mingfeng, Sun Qiaoling

Institute of Physiology, Shandong University School of Medicine.Jinan 250012,Shandong；China

Abstract

To investigate the changes in bcl-2、bax expression and neuron apoptosis in the hippocampus after the blockade of cervical lymphatics, the model of lymphostatic encephalopathy was established by occluding and removing both the superficial and deep cervical lymph nodes in rats. The animals were sacrificed at 1, 2, 3, 5, 7 and 14 days after operation. H＆E staining was used to observe the structure of brain tissues and TUNEL staining was used to detect in situ cell apoptosis in the hippocampus. The expression of bcl-2 and bax in the hippocampus were examined by RT-PCR. The results showed that cerebroedema appeared at day 2 and was most serious at day 5 after the blockade of cervical lymphatics. The number of TUNEL positive cells began to increase at day 2 and reached a maximum at day 5. The expression of bax began to increase at day 1 and reached a maximum at day 2. The expression of bcl-2 began to decrease at day 1 and dropped to minimum at day 5. The items mentioned above recovered to control level at day 14. These results suggested that lymphostatic encephalopathy following the blockade of cervical lymphatics resulted in changes in bcl-2 and bax expression in the hippocampus and that apoptosis was the main form of neuron death.

Key words: Rats, Wistar;Lymph node excision;Brain injuries;Apoptosis;Genes, bcl-2 ;

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引用本文：

李育娴, 夏作理, 陈连璧, 叶文静, 杨明峰, 孙巧玲. 大鼠颈部淋巴引流阻断后海马bcl-2、bax表达和细胞凋亡的动态变化[J]. 生理学报 2005; 57 (1): .

Li Yuxian, Xian Zuoli, Chen Lianbi, Ye Wenjing, Yang Mingfeng, Sun Qiaoling. The changes of bcl-2、bax expression and neuron apoptosis in the hippocampus after the blockade of cervical lymphatics of rats. Acta Physiol Sin 2005; 57 (1): (in Chinese with English abstract).