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杏仁核注射A#beta#25-35后大鼠脑内细胞周期蛋白、tau蛋白和Bax蛋白的异常表达

洪道俊, 裴爱琳, 孙凤艳, 朱粹青

复旦大学上海医学院医学神经生物国家重点实验室，上海200032

摘要

近年来研究发现，阿尔茨海默病(Alzheimer's disease, AD)病人脑内神经元细胞周期相关蛋白的异常表达与AD相关病理改变存在关联。为探讨分淀粉样蛋白([ramyloid,邹)的毒性作用能否导致成年脑神经元表达细胞周期相关蛋白，以及细胞周期相关蛋白表达与神经损伤之间的关系，我们运用免疫组化、积分光密度分析等方法对Ap 25-35多肤片段单侧杏仁核注射的大鼠脑进行了研究。结果显示，Ap 25-35注射的大鼠脑内除了有与神经纤维缠结相关的磷酸化tau蛋白和凋亡相关蛋白Bax蛋白水平增加外，术后7 d细胞周期相关蛋白cyclin A和cyclin BI蛋白在神经元内异常表达，但术后21 d时cyclin A的表达有所降低，而cyclin BI在脑内神经元中已检测不到;免疫荧光双标结果显示邹25-35注射后7 d的大鼠脑内有较多的cyclin B1和Bax . cyclin BI和磷酸化tau蛋白共存的神经元，而Bax
与磷酸化tau蛋白阳性信号很少共存在同一细胞上。以上结果提示，邹可导致成年脑神经元表达细胞周期相关蛋白，这些神经元可能会通过与Bax相关的凋亡途径死亡，或首先导致与AD神经纤维缠结相关的tau蛋白磷酸化。

关键词： 脑; 分淀粉样蛋白; cyclin A; ; cyclin BI ; tau蛋白; 阿尔茨海默病

Aberrarnt neuronal expression of mitotic protein，tau and Bax in the rat brain after inj ection of A#beta#25-35 1rto the amygdala

HONG Dao- Jun, PEI Ai-Lin, SUN Feng Yan, ZHU Cui- Qing

National Key Laboratory of Medical Neurobiology，Shanghai Medical College of Fudan University，National Key Laboratory of Medical Neurobiology，Shanghai Medical College of Fudan University，

Abstract

Recent evidence indicates that the aberrant neuronal expression of mitotic proteins in Alzheimer's disease(AD) brain may be related to AD pathological changes.To investigate whether the toxicity of p-amyloid protein(A#beta#)induces mitotic proteins expression in adult rat brain，we used im-
munohistoche mical and integral optical density analytic method to analyze the adult rat brains，which had been injected withA#beta#2:一35 into unilateral a mygdala·Results showed that the levels of neurofibrillary tan-
gle(NFT) related phosphorylated tau protein and apoptosis related protein Bax were increased inA#beta#25一35injected rat brains，meanwhile the aberrantly expression of mitotic protein cyclin A and cyclin BI was also detected at 7 d after operation，but the level of cyclin A decreased and cyclin BI disappeared at 21 d.I m munofluorescence double labeling presented that cyclin BI was partially co-localized with Bax or phosphorylated tau protein，whereas Bax and phosphorylated tau protein seldom co-localized.These results suggest that causes mitotic protein expression in adult brain neurons，which may die through apoptosis or may be affected by AD NFT-related tau phosphorylation.

Key words: Brain;(3-amyloid;cyclin A;;cyclin BI;tau;Alzheimer's disease

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引用本文：

洪道俊, 裴爱琳, 孙凤艳, 朱粹青. 杏仁核注射A#beta#25-35后大鼠脑内细胞周期蛋白、tau蛋白和Bax蛋白的异常表达[J]. 生理学报 2003; 55 (2): .

HONG Dao- Jun, PEI Ai-Lin, SUN Feng Yan, ZHU Cui- Qing. Aberrarnt neuronal expression of mitotic protein，tau and Bax in the rat brain after inj ection of A#beta#25-35 1rto the amygdala. Acta Physiol Sin 2003; 55 (2): (in Chinese with English abstract).