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蛋白激酶C对大鼠支气管平滑肌Kv通道的影响

刘先胜, 徐永健, 张珍祥, 倪望, 陈仕新

华中科技大学同济医学院附属同济医院呼吸内科，武汉430030

摘要

用全细胞膜片钳、Western印迹法和逆转录一PCR技术，观察蛋白激酶C (protein kinase C, PKC)对大鼠支气管平滑肌细胞(bronchial smooth muscle cells , BS MCs)电压依赖性延迟整流钾通道(Kv)活性及其亚型K,1.5表达的影响。结果为:(1) PKC激活剂豆范酞佛波醇乙酷(phorbol 1 2- myristate 1 3-acetate , P MA)显著抑制急性分离大鼠
BS MCs的K、通道电流，该效应被PKC阻断剂Ro31-822。显著抑制;(2) P MA显著抑制体外培养大鼠BS MCs的Kvl . 5 m RNA和蛋白质的表达，该效应被Ro31-822。显著抑制。上述观察结果提示，PKC活化可抑制大鼠BS MCs的K、通道电流活性，下调KVl . 5亚型的表达水平。

关键词： 支气管; 平滑肌细胞; 电压依赖性延迟整流钾通道; 蛋白激酶C

Effect of protein kinase C on Kv channel in rat bronchial smooth muscle

LI U Maxr Sheng, XU Yong Jian, ZHANG Zheir Xiang, NI Wang, CHEN Shi-Xin

Department of Respiratory Medicine，Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430030

Abstract

The effect of protein kinase C(P KC) signaling pathway on the activity of voltage- dependent delayed rectifier potassium channel(Kv) and the expression of Kv isoform Kvl·5 in rat bronchial smooth cells(BS MCs) were investigated with whole-cell patch clamp，Western blot and RT-PCR techniques .The results showed:(1)phorbol 12- myristate 13-acetate(P MA)，a PKC activator，caused a
significant inhibition of Kv channel currents in rat BS MCs .The inhibition was partly abolished by Ro318220，a PKC inhibitor.(2) P MA caused a significant suppression of the expression of Kvl·5 m RNA and protein in rat BS MCs .These effects were attenuated by Ro31一8220 .The results suggest that in rat BS MCs P KC activation inhibits Kv currents and down regulates the expression of Kvl 5.

Key words: bronchus;Smooth muscle cells;voltage-dependent delayed rectifier potassium channel;protein kinase C

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引用本文：

刘先胜, 徐永健, 张珍祥, 倪望, 陈仕新. 蛋白激酶C对大鼠支气管平滑肌Kv通道的影响[J]. 生理学报 2003; 55 (2): .

LI U Maxr Sheng, XU Yong Jian, ZHANG Zheir Xiang, NI Wang, CHEN Shi-Xin. Effect of protein kinase C on Kv channel in rat bronchial smooth muscle. Acta Physiol Sin 2003; 55 (2): (in Chinese with English abstract).