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银杏苦内酯B对豚鼠模拟缺血心室肌细胞L-型钙电流及胞内游离钙的影响

张志雄, 祁小燕, 徐有秋

上海中医药大学生理教研室，上海200032

摘要

应用全细胞膜片钳及激光共聚焦技术，研究银杏苦内酷B(ginkgolide B , GB)对豚鼠心室肌细胞L -型钙电流及胞内游离钙的作用，并探讨GB心肌保护作用的机制。实验结果显示，在指令电压为。mV时，GB对生理状态下豚鼠心室肌细胞L-型钙电流无明显作用。在模拟缺血状态下，L-型钙峰值电流减小37. 71%，但加入1 umol/ L GB后，可逆转缺血引起的L-型钙电流的降低，与缺血对照组比较，有显著性差异(P < 0. 05) 0 1 umol/ L GB能使由于模拟缺血而上移的L-型钙电流电压曲线回复正常。在生理状态下，0. 1 . 1 . 10 mol/ L GB分别使心肌细胞内游离钙降低10.58(n=12),17.27 %(n一12)1 6.35 % ( n =10)，与对照组相比有非常显著性差异。模拟缺血液灌流12 min时，细胞内游离钙浓度增加20.15，在模拟缺血液中分别加入1 umol/ L nifedipine或5 mmol/L NiClz，结果显示:模拟缺血液灌流12 min，与正常对照组相比细胞内钙分别增加18.18%(P>0.05)与11 %(P<0.05)。在模拟缺血液中加入1 mol/ L GB灌流12 min时细胞内钙仅增加9.60 % ( n一12,P<0.001)，与缺血对照组相比有显著性差异(P<0. 05)。结果表明，GB可逆转模拟缺血造成L-型钙电流的降低，同时可部分减轻由于缺血所造成的细胞内钙的超载。

关键词： 银杏苦内酷B; 心室肌细胞; 缺血; L-型钙电流; 细胞内游离钙

Effect of ginkgolide B on L type calcium ctrrernt and cytosolic [ Ca~(2十)]i in gtunea pig ischemic verntricular myocytes

ZHANG Zhi-Xiong, QI Xiao- Yan, XU You Qiu

Department of Physiology, Shanghai University of Traditional Chinese Medicine , Shanghai 200032；Department of Physiology, Shanghai Second Medicial University, Shanghai 200025

Abstract

With whole-cell variant patch-clamp and laser scanning confocal microscope technique，we examined the effect‘ginkgolide B(GB) from ginkgo leaves on L -type calcium current and cytosolic[Ca 2十]，in guinea pig ische mic ventricular myocytes·The results showed that under normal conditions，at a test voltage of 0 m V，GB had no significant effect on ICa,L;and during ische mia，the peak Cat十current reduced by 37. 71%，and the 1- V curve of ICa,L was shifted upward·1 p mol/ L GB reversed the change induced by ische mia，a result being significantly different from those of the ishemia group(P<0.05).Under control condition，0. 1 ,1 ,10 umol/ L GB decreased intracellular calcium concentration by 10. 58%，17.27%and 16. 35%(n一12，12，10，P<0.01一0.001)，respectively .With perfusion of ische mic solution for 12 min，intracellular calcium concentration increased by 20.15%·After a 12 min-perfusion of ischemic solution containing 1 pmol/ L nifedipine or 5 mmol/
L N 1G2，intracellular calcium concentration increased by 18.18%(P>0. 05 vs ische mia) and 11%(P<0.05vs ische mia)，respectively .After 12 min of perfusion with ische mic solution containing 1 p mol/ L GB，intracellu
lar calcium concentration increased by 9.6%(P<0.05 us ische mia).It is shown that GB could reverse the decrease of ICa,L and partially inhibit calcium overload during ischemia.

Key words: ginkgolide B;ventricular myocytes;ische mia;L -type calcium current;cytosolic[Ca~［(2十)i］

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引用本文：

张志雄, 祁小燕, 徐有秋. 银杏苦内酯B对豚鼠模拟缺血心室肌细胞L-型钙电流及胞内游离钙的影响[J]. 生理学报 2003; 55 (1): .

ZHANG Zhi-Xiong, QI Xiao- Yan, XU You Qiu. Effect of ginkgolide B on L type calcium ctrrernt and cytosolic [ Ca~(2十)]i in gtunea pig ischemic verntricular myocytes. Acta Physiol Sin 2003; 55 (1): (in Chinese with English abstract).