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钠一氢交换蛋白mRNA在人胎儿两个发育阶段中的组织特异性表达

林万敏, 陈献华, 徐容, 刘漩, 徐平

复旦大学生命科学学院脑研究中心，基因生理学研究室，立人实验室，上海200433；华东师范大学生命科学学院，上海200062

摘要

钠一氢交换蛋白(Na~(+)一H~(+) exchangers，NHE)至少包含6个不同的亚型，生长因子可激活其表达。目前，对在发育过程中NHE的表达了解甚少。本文利用RT PCR观察了4种NHE亚型的m RNA在人胎儿的两个不同发育阶段(11周、16周)在不同组织中的表达，以研究它们的发育调控。结果显示，NHE1 mRNA在两种胎龄的多种组织中均有表达。和16周胚胎相比，11周的胚胎的NHE1 m RNA的表达较弱，并且表现出明显的组织差异。据此推测，NHE1的管家(house- keeping)功能可能至少在11周就开始形成，而最迟在16周已基本建立;NHE2和NHE3 mRNA在11周和16周的胚胎组织中的特异性表达呈现相反的变化趋势及组织分布上的重叠，后者与NHE2和NHE3在成人组织中的分布及功能的重叠的特点相吻合;NHE5 mRNA的表达在11周的胚胎组织中比较普遍，而在16周的胚胎组织中则局限在小脑组织中。本研究表明，在人胚胎发育的11一16周期间，NHE的组织特异性表达表现出时间依赖性的调控，而在
不迟于胚胎发育的第16周，具有“管家功能”的NHE1的基因表达已与成人相似。

关键词： 生理学; 基因表达; RT PCR; Na~(+)-H~(+)交换蛋白

Tissue specific expression of、Na~(+)-H~(+) exchanger isoformas at two developmental stages of human fetus

LIN Wair Mn, CHEN Xiair Hua, XU Ro, LI U Xuan, XU Ping

'Laboratory of Genomic Physiology and Center for Brain Research, Liren Laboratory, School of Life Sciences Fundan University, Shanghai 200433；School of Life Sciences , China Easten Normal University, Shanghai 200062

Abstract

Na~(十)-H~(十)exchangers(NHE) are major membrane proteins that have been identified as signal transduction mediators in the regulation of cell differentiation and important me mbrane ion transporters in the regula-
tion of the intercellular pH and the cell volume. NHE are composed of at least six isoforms and activated in growth factor regulated cell rentiation.However，little is known about the differential regulation of NHE expression in the development·In the present study，we studied developmental regulation of the expression of NHE isoforms in human fetal tissues by comparing the expression of various isoforms between two developmental stages，i. e.，week 11(11w and week 16(16w .The results demonstrated that NHEl transcripts were ex-
pressed ubiquitously .In comparison to the expression at 16 W，the level of NHEl transcripts was low and varied significantly in a tissue-specific pattern at 11 W，suggesting that the“house-keeping" function of MHEl occurs at
11 W or earlier and becomes well established at least as early as at 16 W.The tissue-specifically restricted expression of NHE2 and NHE3 was regulated at 11 W and 16 W in an opposite tendency，supporting the overlapping relationship between NHE2 and NHE3 in the tissue distribution as reported in adults .NHE5 expression was relatively ubiquitous at 11 W and became restricted in the cerebellum at 16 W，suggesting that the restrictive expression of NHE5 in the brain occurs later than that of other isoforms .The present study demonstrates a space time-dependent regulation of the tissue-specific expression pattern of NHE isoforms during human development between 11 W and 16 W.The results also suggest that at 16 Wor earlier the expression pattern of developing tis-
sues becomes similar to that of adult tissues.The observed developmental regulation of NHE expression provides a molecular basis for further study of the function and regulation of NHE gene during development.

Key words: Physiology;Gene expression;RT PCR; Na~(十)-H~(十)exchangers

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引用本文：

林万敏, 陈献华, 徐容, 刘漩, 徐平. 钠一氢交换蛋白mRNA在人胎儿两个发育阶段中的组织特异性表达[J]. 生理学报 2003; 55 (1): .

LIN Wair Mn, CHEN Xiair Hua, XU Ro, LI U Xuan, XU Ping. Tissue specific expression of、Na~(+)-H~(+) exchanger isoformas at two developmental stages of human fetus. Acta Physiol Sin 2003; 55 (1): (in Chinese with English abstract).