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结核分枝杆菌感染对人巨噬细胞离子通道及其调控元件转录表达的影响

谢建平, 李瑶, 乐军, 徐永忠, 梁莉, 胡昌华, 于善谦, 王洪海

复旦大学生命科学学院遗传学研究所病原生物学组遗传工程国家重点实验室，上海200433；生海市肺科医院，上海200433

摘要

为研究人巨噬细胞的离子通道及其调控元件是否参与了抗结核分枝杆菌感染免疫，利用表达谱芯片技术研究细菌感染后宿主巨噬细胞基因的表达情况。在全局表达谱分析的基础上，重点分析了离子通道及其调控元件的表达，并比较无毒株和临床分离有毒株在诱导离子通道及其调控元件表达方面的差异。结果表明，细菌感染影响离子通道及其调控元件基因的表达，涉及的离子通道包括钾通道、钠通道、氯通道、钙通道，差异表达的调控元件包括G蛋白、G蛋白偶联受体、蛋白质激酶和磷酸化酶;临床株感染影响的离子通道及其调控元件较无毒株广泛和丰富。这些观察结果提示，离子通道及其调控元件参与了宿主细胞对感染细菌的免疫应答，有关离子通道及其调控元件在抗结核免疫中的作用有待进一步研究。芯片研究的结果为将来的研究提供了线索。

关键词： 离子通道; 受体; 结核分枝杆菌; 巨噬细胞; 表达谱芯片

Effects of Mycobacterium tuberculosis infection on the transcriptional expression of human macrophage gene encoding ion channels andrelated regulatory elements

XIE Jian Ping, LI Yao, YUE Jun, XU Yong Zhong, Liang Li, Hu Changhua, YU Shan Qian'，WANG Hong Hai

State Key Laboratory of Genetic Engineering Section of Pathogen Biology, Institute of Genetics School of life Science Fuda University, Shanghai 200433；United Gene Holdings , Ltd. , Shanghai 200092；Shanghai Pulmonary Hospital, Shanghai 200433；Institute of Modern Biopharmaceuticals，School of Life Science，South west China Normal University，Chongqing 400715

Abstract

Expression microarray was employed in this study to investigate whether the ion channels and their regulatory ele ments encoding genes participate in the immune response to Mycobacteriu m tuberculosis infection .The results of a virulent strain were compared with those of the clinically isolated trains .The data demonstrate that K~(+)，Na~(+)，Ca~(+)and Cl一channels and their regulatory elements，such as the G protein，receptor and second messenger，protein kinase and protein phosphatase were involved in the immune reaction.The clinical strain affected more types of ion channels and respective regulatory elements·The data provides clues for further scrutiny into the role of ion channels and related elements in the interaction between Mycobacte riu。tuberculosis and host macrophage.

Key words: Ion channel;Receptor;expression microarray;macrophage U937;Mycobacte ri u。tuberculosis

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引用本文：

谢建平, 李瑶, 乐军, 徐永忠, 梁莉, 胡昌华, 于善谦, 王洪海. 结核分枝杆菌感染对人巨噬细胞离子通道及其调控元件转录表达的影响[J]. 生理学报 2003; 55 (1): .

XIE Jian Ping, LI Yao, YUE Jun, XU Yong Zhong, Liang Li, Hu Changhua, YU Shan Qian'，WANG Hong Hai. Effects of Mycobacterium tuberculosis infection on the transcriptional expression of human macrophage gene encoding ion channels andrelated regulatory elements . Acta Physiol Sin 2003; 55 (1): (in Chinese with English abstract).