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大鼠二叉神经脊束间质核接受内脏和躯体伤害性信息的calbindin D-28k神经元向臂旁核的投射

马文领, 张文斌, 张宇飞

第四军医大学基础医学部解剖学教研室，梁钵锯脑研究中心，西安710032

摘要

应用荧光金(F G)逆行束路追踪结合F os和calbindin D28k(CB)免疫荧光组织化学三重标记法，观察了大鼠三叉神经脊束间质核(INV)接受口面部皮肤和上消化道伤害性信息的CB神经元向臂旁核(PB)的投射。结果显示，口周刺激组FG逆标细胞和F os免疫反应阳性细胞主要分布于注射和刺激同侧INV的背侧边缘旁核(Pa Md)和三叉旁核(Pa V);大量的CB免疫阳性细胞分布于双侧INV。同侧INV内FG逆标细胞中有77. 3%呈CB免疫反应阳性，40. 7%呈F os免疫反应阳性。在FG和CB双标记的神经元中，又有一部分(约38.5)为FG/ CB/ Fos三标细
胞。上消化道刺激组的FG逆标细胞、CB免疫阳性细胞和F G/ CB双标细胞的数量和分布与口周刺激组相似，但F os免疫阳性细胞分布于双侧的INV。在同侧INV, FG/ Fos双标细胞占FG逆标细胞总数的41.9%, FG/CB/F，三标细胞占F G/ CB双标细胞的52. 0。以上结果提示，INV直接投射到PB的CB神经元接受口面部皮肤和上消化道的伤害性信息，CB神经元可能参与经INV中继的外周伤害性信息向PB的传递。

关键词： 神经生物学; 三叉神经脊束间质核; 免疫组织化学; 臂旁核; 伤害性信息; calbindin D 28k (CB) ; 大鼠

Calbindin D28K containing neurons receivirng visceral and somatic nociceptive information in interstitial nucleus of the spinal trigeminal tract project to the parabrachial nuclei in the ratt

MA Weir Ling, ZHANG Weir Bin, ZHANG YuFei

Department of Anatomy and K. K. Leung Brain Research Centre，the Fourth Military Medical University，Xi an 710032-

Abstract

The calbindin D 28 k(CB)一containing neurons in the interstitial nucleus of the spinal trige minal tract(INV) that receive visceral and orofacial somatic nociceptive information and emanate projections to the parabrachial nuclei(PB) were investigated by the triple-labeled methods of fluorogold
(FG) retrograde tracing combined with Fos and CB proteins immunofluorescence histochemistry in the rat .The results showed(1)in the perioral stimulation group，a large number of FGretrograde labeled and Fos-i m munoreactive neurons were found in the paratrige urinal nucleus(Pa V) and the dorsal para-
marginal nucleus(PaMd) of the INV ipsilateral to FG and formalin injection made to the PB and lips，respectively，while a lot of CB-immunoreactive neurons were distributed in the INV bilaterally;(2) amajority of the FGretrograde labeled neurons(77. 3%)were double-labeled with CB，and 40. 7%of
them were double-labeled with Fos;about 38.5%of FG/ CB double-labeled neurons were FG/ CB/ Fostriple-labeled in the INV;and(3) in the upper alimentary tract stimulation group，the distribution and the numbers of FG-retrograde labeled，C& i m munoreactive neurons and F G/ CB double-labeled neurons in
the INV were similar to those of the perioral stimulation group as described above，except that the Fos immunoreactive neurons were distributed in the INV bilaterally，approximately 41·9%of the FGretrograde labeled neurons were FG/ Fos double-labeled，and over half(52. 0%)of those double-labeled neurons were FG/ CB/ Fos triple-labeled.The results indicate that a part of CB-containing neurons in the INV receive orofacial somatic and visceral nociceptive information and that these neurons sent projections directly to the PB .The C什containing neurons might play an important role in the transmission of the
peripheral nociceptive information from INV to PB.

Key words: eurobiology;interstitial nucleus of the spinal trige urinal tract;im munohistoche mistry; parabrachial nuclei;nociceptive information;calbindin仆28k;rat

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引用本文：

马文领, 张文斌, 张宇飞. 大鼠二叉神经脊束间质核接受内脏和躯体伤害性信息的calbindin D-28k神经元向臂旁核的投射[J]. 生理学报 2003; 55 (1): .

MA Weir Ling, ZHANG Weir Bin, ZHANG YuFei. Calbindin D28K containing neurons receivirng visceral and somatic nociceptive information in interstitial nucleus of the spinal trigeminal tract project to the parabrachial nuclei in the ratt. Acta Physiol Sin 2003; 55 (1): (in Chinese with English abstract).