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白细胞介- 2引起离体大鼠主动脉环舒张及其作用机制

曹春梅, 叶松, 俞虎, 徐庆生, 叶治国, 沈岳良, 陆源, 夏强.

浙江大学医学院生理学教研室，杭州310031

摘要

研究白细胞介素一2 (interleukirr2 , IL 2)对离体大鼠胸主动脉环收缩张力的作用及其可能机制。采用累积加药法，检测IL 2对去氧肾上腺素(P助和KCl预收缩的胸主动脉环收缩张力的影响。结果表明，IL-2 (1、10 100 1 000 U/ MI)对PE (10 umol/ L)预收缩的内皮完整血管环产生浓度依赖性的舒张作用，而对KCl (120 m mol/L)预收缩的血管无作用。去除内皮后，IL-2的舒张作用被取消。用一氧化氮合酶抑制剂L- NAME (0.1 mmol/L)和
鸟普酸环化酶抑制剂亚甲蓝(10 umol/ L)预处理，均可阻断IL-2的舒张血管作用。用环氧合酶抑制剂吲哚美辛(Indo,10 umol/L)预处理可阻断IL-2的血管舒张作用。从上述观察结果推论，IL-2通过NO-鸟普酸环化酶和环氧合酶途径产生内皮依赖的血管舒张作用。

关键词： 白细胞介素一2; NO; 胸主动脉环; 内皮

Interleukirr 2 induced endothelium dependent relaxation of rat thoracic aorta

CAO Chuir Mei, YE Song, YU Hu, XU Qing Sheng, YE Zhi- Guo, SHEN Yue-Liang, LU Yuan, XIA Qiang

Department of Physiology，Zhejiang University School of Medicine，Hangzhou 310031

Abstract

Interleukixr 2(IL 2) therapy often results in potentially life-threatening side effects including hypotension .However，the mechanism has not been completely elucidated.In order to determine whether IL -2 modifies vascular tone，we investigated the effect of IL -2 on rat thoracic aorta rings and the underlying mechanisms.Effects of IL -2 on the contraction of high KCl and phenylephrine(PE) preconstricted rat thoracic aorta with or without endothelium were determined by organ bath technique .To explore the mechanism，nitric oxide synthase inhibitor L一N( G)一nitroarginine methyl ester(L- NAME)，
guanylyl cyclase inhibitor methylene blue，and cyclooxygenase inhibitor indomethacin were used .IL 2(10一1000 U/ ml) caused concentration dependent relaxation of aorta rings preconstricted with PE(10p mol/ L) in endothelium-intact rings，but had no effect on KCl(120 m mol/ L) preconstricted rings.Re-
moval of the endothelium，or pretreatment with L- NAME(0. 1 m mol/ L) or methylene blue(10 umol/L) or indomethacin(10 pmol/L)，inhibited the relaxation of IL -2.The results indicate that the relaxation by IL -2 in rat aorta ring is endothelium7dependent and is possibly mediated by the NO-guanylyl cy-
clase pathway and cyclooxygenase-dependent pathway.

Key words: interleukin-2;NO;;

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引用本文：

曹春梅, 叶松, 俞虎, 徐庆生, 叶治国, 沈岳良, 陆源, 夏强.. 白细胞介- 2引起离体大鼠主动脉环舒张及其作用机制[J]. 生理学报 2003; 55 (1): .

CAO Chuir Mei, YE Song, YU Hu, XU Qing Sheng, YE Zhi- Guo, SHEN Yue-Liang, LU Yuan, XIA Qiang . Interleukirr 2 induced endothelium dependent relaxation of rat thoracic aorta. Acta Physiol Sin 2003; 55 (1): (in Chinese with English abstract).