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性腺甾体激素对雌、雄大鼠下丘脑血管升压素mRNA水平的影响

戴文捷, 陆利民, 姚泰

(上海医科大学生理学教研室，上海200032)

摘要

实验用10-11周龄经阉割的雌、雄Sprague-Dawley大鼠进行。以3'末端异经基洋地黄毒甙标记的26个碱基长的寡核昔酸作为检测探针，用核酸斑点杂交技术检测大鼠下丘脑血管升压素(vasopressin, AVP) mRNA水平。在假手术对照组，雄性大鼠下丘脑AVP mRNA水平比雌性大鼠高4500 (P<0.05), 1M足渗透压高于雌性大鼠(P<0. 05)。摘除卵巢的大鼠下丘脑AVPmRNA水平比假手术组雌性大鼠高30写(P<0. 05)。摘除皋丸的大鼠下丘脑AVP mRNA水平低于假手术组雄性大鼠，但其差别尚无统计学显著意义。阉割的雌、雄大鼠血浆渗透压无显著性差别。在阉割的雄性大鼠中，皮下注射雌二醇可降低下丘脑AVP mRNA水平((P<0. 05)，而给予攀丸酮、孕酮及雌二醇与孕酮的混合物则没有作用。给摘除卵巢的雌性大鼠皮下注射雌二醇或雌二醇与孕酮的混合物都可使下丘脑AVP mRNA水平下降((P<0. 01)，而注射肇丸酮或单独给予孕酮都没有作用。皮下注射各类性激素后，血浆渗透压与对照组无显著差别。这些结果提示，性腺甾体激素可影响大鼠下丘脑内AVP mRNA的水平。

关键词： 血管升压素; 信使核糖核酸; 性激素; 性腺摘除

EFFECTS OF GONADAL STEROID HORMONES ON HYPOTHALAMIC VASOPRESSIN mRNA LEVEL IN MALE AND FEMALE RATS

DAI WEN-JIE, LU Li-Min, YAo TAI

Department of Physiology, shanyhai Medical University, Shanghai 200032)

Abstract

Experiments were carried out in 10-11一week old gonadectomized male and fe-
male Sprague-Dawley rats. Dot-blot analysis and 31一end digoxigenin-labeled 26 meroligonucleotide probe was used in detecting the mRNA level hypothalamic vasopressin (AVP).The basal hypothalamic AVP-mRNA level in the sham-operated intact males was 4 5%higher than that in the sham-operated intact females (P<0. 05).Plasma osmolality was also higher in the sham-operated intact males than in the sham-operated intact females (P<0. 05).The hypothalamic AVP-mRNA level in ovariectomized rats was 30%higher than that in sham-operated intact females (P< 0. 05).Although the hypothalamic AVP mRNA level tended to be lower in castrated males than in sham-opcrated intact males，the difference was not statistically significant. The difference in plasma osmolality between gonadectomized males and females was statistically insignifcant. In castrated males，hypothalamic AVP-mRNA level was decreased following sc injection of estradiol(P<0.05)，but testosterone，progesterone or a combination of
estradiol and progesterone were without effect.

Key words: Vasopressin;;mRNA;

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引用本文：

戴文捷, 陆利民, 姚泰. 性腺甾体激素对雌、雄大鼠下丘脑血管升压素mRNA水平的影响[J]. 生理学报 1996; 48 (6): .

DAI WEN-JIE, LU Li-Min, YAo TAI. EFFECTS OF GONADAL STEROID HORMONES ON HYPOTHALAMIC VASOPRESSIN mRNA LEVEL IN MALE AND FEMALE RATS. Acta Physiol Sin 1996; 48 (6): (in Chinese with English abstract).