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糖皮质激素对谷氨酸和GABA受体的电生理反应的快速调制作用

汪文, 邢宝仁, 陈宜张

第二军医大学生理教研室，神经科学研究所，上海200433)

摘要

在大鼠下丘脑薄片和豚鼠腹腔神经节上，分别用玻璃微电极细胞外和细胞内记录方法，观察了10-s mol/L糖皮质激素(GC)对谷氨酸和GABA受体介导效应的快速调制作用。结果表明，GC灌流后5 min，对谷氨酸受体介导的效应起抑制作用，而对GABA受体介导的效应起增强作用。撤除GC后，神经元对谷氨酸和GABA的反应恢复到对照水平。低钙高镁灌流液不能取消GC的调制作用。结果提示，GC在不需要突触环路条件下，可能通过非基因组途径影响谷氨酸和GABA受体介导的效应。

关键词： 糖皮质激素; 下丘脑薄片; 腹腔神经节; 谷氨酸; GABA受体; 调制作用

FAST MODULATION OF GLUTAMATE AND GABA RECEPTORMEDIATE ELECTROPI-IYSIOLOGICAL RESPONSES BY GLUCOCORTICOID

WANG WEN, XING BAO-REN , CHEN YI-ZHANG

(Institute of Neuroscience, Department of Physiology，Second Military Medical University, Shanghai 200433)

Abstract

Fast modulation by glucocorticoid on gluatmate and GABA receptor-mediated electrophysiological responses was investigated by extracellular iontophoresis and intracellular techniques in rat hypothalamic brain slices and guinea pig coeliac ganglion preparations respectively. The results were as follows:Glucocorticoid inhibited the glutamate receptor-mediated excitatory response and potentiated the GABA receptor-mediated inhibitory response within 5 min after perfusion. All these responses are reversible after removal of the glucocorticoid. Low Cat*- high Mgz+ medium was ineffective to block the fast modulatory effects of glucocorticoid on the gluatmate and GABA receptor-medi-
ated responses. It appears that the latter responses are mediated through some non-genomic mechanism without an involvement of synaptic circuitry.

Key words: Glucocorticoid;Hypothalamic slice;coeliac ganglion;Glutamate;GABA;

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引用本文：

汪文, 邢宝仁, 陈宜张. 糖皮质激素对谷氨酸和GABA受体的电生理反应的快速调制作用[J]. 生理学报 1996; 48 (6): .

WANG WEN, XING BAO-REN , CHEN YI-ZHANG. FAST MODULATION OF GLUTAMATE AND GABA RECEPTORMEDIATE ELECTROPI-IYSIOLOGICAL RESPONSES BY GLUCOCORTICOID. Acta Physiol Sin 1996; 48 (6): (in Chinese with English abstract).