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脊髓中血管紧张素Ⅱ的释放及其抗电针镇痛

沈上, 李君, 王晓民, 韩济生

(北京医科大学神经科学研究中心，北京100083)

摘要

采用放射免疫分析方法测定不同频率电针引起大鼠脊髓液中血管紧张素正(AⅡ)免疫
活性(it )的变化，并观察阿片受体在其中起的作用。结果表明:(1) 2 Hz电针时大鼠脊髓灌流液中A Ⅱ< -it减少200o，但无充计学意义;15 Hz电针使灌流液中A Ⅱ -it降低620o(P<0.01); 100Hz时A Ⅱ -Ⅱ:增加600o (P<0.05)0 (2)脊髓蛛网膜下腔((Ⅱ. t.)注射阿片受体拮抗剂纳洛酮后15 Hz电针反使灌流液中A Ir含量增加12500 (P<0.05)0 (3)特异性#mu#受体激动剂ohmefentanyl 可以抑制A Ⅱ(释放，s和K激动剂无效。(4)i.t.注射All受体拮抗剂saralasin明显加强100 Hz 电针镇痛((P<0. 05)，但对2 Hz和15 Hz电针针效影响不大。结果提示:AB拮抗剂与电针合用可能有助于加强100 Hz电针的镇痛效果。内源性阿片肽通过#mu#受体抑制电针期间A Ⅱ的释放。

关键词： 血管紧张素Ⅱ; 放射免疫分析; 电针镇痛; 电针频率; 阿片受体; 脊髓

ANGIOTENSIN Ⅱ RELEASE AND ANTI-ELECTROACUPUNCTURE ANALGESIA IN SPINAL CORD

SHEN SHANG, LI JUN, WANG XIAO-MIN AND HAN JI-SHENG

(Neuroscience Research Center, Beijing Medical University, Beijing 100083)

Abstract

Changes in the content of angiotensin Ⅱ( (A Ⅱ)immunoreactivity (it)in rat
spinal perfusate induced by electroacupuncture (EA) stimulation of different frequencies were measuerd by radioimmunoassay (RIA).The results were analyzed in relation to the role of opioid recepor.(1)2 Hz EA produced a 20%(P>0. 05) decrease in A Ⅱ -it content in the spinal perfusate o 15 Hz EA produced an even more decrease (62%，P<0. O l)，whereas 100 Hz produced a significant increase(65%，P<0. 05).(2) The release in spinal A Ⅱ一it produced by 15 Hz EA was reversed by the opioid antagonist naloxone to a of 125%highter than that of the control (PLO. 05)，suggesting that 1.5 Hz EA may accelerate the release of endogenous opioids to suppress the release of A Ⅱ.(3) This was substantiated by the finding that intrathecal (i. t.)injection of the selective mu agonist ohmefentanyl produced a dramatic suppression(200a，
P< 0. 05) of A Ⅱ release，but not by delta and kappa agonist. (4)Intrathecal injection of salarasin，the angiotensin receptor antagonist，produced a significant potentiation of the analgesia produced by 100 Hz EA，but not that produced by 2 or 15 Hz EA. It is concluded that 15 Hz EA may induce the release of endogenous opioids acting on p opioid receptor so as to suppress A If release，and that 100 Hz EA may acceleate the release of A Ⅱ servinp- as a brake for 100 Hz EA-induced analpensia.

Key words: angiotensin Ⅱ;electroacupuncture analgesia;frequency;immunoreactivity;Opioid receptor;spinal cord

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引用本文：

沈上, 李君, 王晓民, 韩济生. 脊髓中血管紧张素Ⅱ的释放及其抗电针镇痛[J]. 生理学报 1996; 48 (6): .

SHEN SHANG, LI JUN, WANG XIAO-MIN AND HAN JI-SHENG. ANGIOTENSIN Ⅱ RELEASE AND ANTI-ELECTROACUPUNCTURE ANALGESIA IN SPINAL CORD . Acta Physiol Sin 1996; 48 (6): (in Chinese with English abstract).