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反义寡核昔酸片段可抑制鲫鱼视网膜电压依赖性钾离子通道的表达

包永德, 朱辉, 朱幸

(中国科学院上海生理研究所视网膜研究室，上海200031)

摘要

爪蟾卵母细胞经注射卿鱼(Carassius carassius)视网膜总RNA后，可表达大量电压依赖性钾离子通道。在此基础上，我们进一步发现，一个特定序列的寡核昔酸能专一地抑止该通道的装达。由于我们设计与合成的该片段与果蝇、小鼠中已克隆的钾通道中编码N`端的一个多肽的mRNA完全互补，因此推测:鲫鱼视网膜中的K~(十)通道基因这个区域与其它物种的K~(十)通道有高度同源性，这为克隆该基因和研究它的功能提供了重要资料。

关键词： 爪蟾卵母细胞; 卿鱼视网膜RNA表达; 反义寡核昔酸片段; 电压依赖性钾离子通道

REPRESSION OF VOLTAGE GATED K+ CHANNEL EXPRESSED IN XENOPUS OOCYTES AFTER INJECTION TOTAL RNA FROM CARP RETINA BY AN ANTISENSE OLIGONUCLEOTIDE

BAO YONG-DE, Zhu Hui, ZHU XING

(Laboratory of Retina Research, Shanghai Institute of Physiology, Chinese Academy of Sciences, Shanghai 200031)

Abstract

Injection of total RNA from carp retina induced the formation of a membrane K十channel in Xenopus oocytes. We found an oligonucleotide complementary to the mRNA coding for a peptide fragment in the K+ channel of drosophila and rat could sulpress the K+ channel current in a very specific manner. This provides important infomation for the cloning of this gene and for studying it's function.

Key words: oocytes of xenopus laevis;expression of RNA from carp retina; antisense oligonL cleotide;

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引用本文：

包永德, 朱辉, 朱幸. 反义寡核昔酸片段可抑制鲫鱼视网膜电压依赖性钾离子通道的表达[J]. 生理学报 1996; 48 (6): .

BAO YONG-DE, Zhu Hui, ZHU XING. REPRESSION OF VOLTAGE GATED K+ CHANNEL EXPRESSED IN XENOPUS OOCYTES AFTER INJECTION TOTAL RNA FROM CARP RETINA BY AN ANTISENSE OLIGONUCLEOTIDE . Acta Physiol Sin 1996; 48 (6): (in Chinese with English abstract).