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NA和5-HT对小脑脑片浦肯野细胞 自发及诱发电活动的影响

胡卫红, 王建军, 张敏跃, 余启祥

南京大学生物科学与技术系生理教研室，南京210093)

摘要

在大鼠小脑脑片上观察了NA和5-HT对浦肯野细胞(PC)的自发放电活动及由白质刺激所
引起的诱发放电活动的影响。结果表明:(1) NA使PC产生抑制、兴奋和双相反应，以抑制反
应为主(79.80o); 5-HT引起PC兴奋和抑制反应，以兴奋反应略多(57.800)0 (2)先后灌流NA
和5-HT对同一个PC自发放电的影响主要为抑制(NA)一兴奋((5-HT) (53.8%). (3) NA对PC
的诱发复杂锋电位(CS)和简单锋电位(SS)反应，主要产生增强效应((57.1%和62.80o); 5-HT对PC诱发CS和SS反应则主要产生压抑作用(60. 0%和68.200)0 (4)先后灌流NA和5-HT对
同一个PC的诱发CS和SS反应，主要表现为NA对这两种诱发反应的增强和5-HT的压抑效应
(60. 0%和52.900)。这些结果提示，N人能和5-HT能传入纤维可以通过释放NA和5-HT调节
PC的兴奋性水平并改变PC对爬行纤维和苔状纤维突触传入的反应敏感性，影响小脑皮层神经元网络的感觉运动整合过程。

关键词： 小脑皮层; 浦肯野细胞; 去甲肾上腺素; 5-经色胺; 小脑胺能纤维传人系统; 小脑脑片

EFFECTS OF NA AND 5一HT ON THE SPONTANEOUS AND EVOKED ACTIVITIES OF PURKINJE CELLS IN CEREBELLAR SLICE

HU WEI-HONG, WANG Jian-Jun, ZHANG MIN-YUE, YU QI-XIANG

Division of Physiology, Department of Biological Science and Technology, Nanjing University, Nan.jing 210093)

Abstract

Effects of NA and 5-HT perfusion on the spontaneous activity and evoked responses of the Purkinje cells (PCs) to stimulation of white matter were studied in the rat cerebellar slices. The results were as follows:(1)Perfusion with NA could elicit inhibitory，excitatory or biphasic responses of the spontaneously discharging PCs，with inhibitory response in dominance (79. 8%).5-HT perfusion could also exert either excitatory or inhibitory effect on PC spontaneous activity.(2)When NA and 5-HT were perfused successively，NA tended to inhibit while 5-HT tended to excite in 53. 8%of the tested cells.(3)Most of the complex spike (CS) and simple spike (SS) responses evoked by white matter stimulation could be potentiated by NA(68. 2%，57. 1%)or depressed by 5-HT(60. 0%，68. 2%).(4)On the same cell under successively perfusion with NA and 5-HT，the evoked CS and SS responses of most cells(60. Ooo，52. 9%)showed a tendency to be facilitated by NA and suppressed by 5-HT. These results suggest that aminergic afferent fibers，by releasing NA and 5-HT，may affect
PC's excitability and responsiveness to synaptic inputs from mossy and limbing fibers，thus playing an important role in the cerebellar function.

Key words: Cerebellar cortex;Purkinje cell;Noradrenaline;5-hydroxytrypamine;cerebellar aminergic afferent system;cerebellar slice

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引用本文：

胡卫红, 王建军, 张敏跃, 余启祥. NA和5-HT对小脑脑片浦肯野细胞 自发及诱发电活动的影响[J]. 生理学报 1996; 48 (6): .

HU WEI-HONG, WANG Jian-Jun, ZHANG MIN-YUE, YU QI-XIANG. EFFECTS OF NA AND 5一HT ON THE SPONTANEOUS AND EVOKED ACTIVITIES OF PURKINJE CELLS IN CEREBELLAR SLICE. Acta Physiol Sin 1996; 48 (6): (in Chinese with English abstract).