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心功能不全大鼠心脏#alpha#_(l)一肾上腺素受体亚型的变化

许开明, 张幼怡, 曲鹏, 张萍, 田斌, 韩启德

(北京医科大学第三医院血管医学研究所，北京100083)

摘要

通过主动脉和左肾动脉缩窄复制心功能不全大鼠模型，并采用放射配基结合实验和半
定量逆转录一聚合酶链反应(RT-PCR)，从蛋白质和基因转录水平上观察在心功能不全时大鼠心脏a，一肾上腺素受体三种亚型的改变。Scatchard分析结果显示，在心功能不全组与对照组，大鼠心脏组织‘251-BE与a,一肾匕腺素受体结合的亲和性(229士32与195士15 pmol/L)和数量(102士12与96士17 fmol/mg)均无显著差异。但WB4101对’251-BE的竞争性抑制实验显示在心功能不全组高亲和性位点所占百分比较对照组显著增高((51士7%与22士50o), RT-PCR的结果显示，在心功能不全大鼠心脏at-AR的mRNA水平高于对照组，但a,H-AR的mRNA水平低于对照组，而a,D-AR的mRNA水平在两组之间没有显著差异。综合上述结果可知，在心功能不全大鼠，心脏#alpha#_(1A)-AR增加，#alpha#_(1B)-AR下降，而#alpha#_(1D)AR则没有显著改变。

关键词： 心功能不全; 心脏; #alpha#_(1)一肾上腺素受体; 受体亚型

ALTERATION OF CARDIAC #alpha#_(l)-ADRENERGIC RECEPTOR SUBTYPES IN CARDIAC INSUFFICIENT RAT

XU KAI-MING, ZHANG YOU-YI , QU PENG, ZHANG PING, Tian Bin, HAN QI-DE (CHIDE HAN)

(Institute of Vascular Medicine, The Third Hospital, Beijing Medical University, Beijing 100083)

Abstract

The cardiac insufficiency was produced by simultaneous constriction of abdominal aortae and renal artery in rat. Radioligand binding and reverse transcription- pol),merase chain reaction (RT-PCR) were used respectively to determine the alterations of cardiac a,-adrenergic receptor (a,-AR) subtypes at protein level and gene transcription level. The Scatchard analysis of 1zsI_BE 2254 saturation curves showed that in cardiac insufficient rat hearts the KD values (229士32 vs 195士15 pmol/L) and the Bm$X vat ues(102士12vs96士17 fmol/mg) were not significantly different from those of control hearts. In the insufficient rat hearts，the inhibition of a,一AR specific binding by
WB 4101 showed that the high affinity sites (a,A+a,) were increased from the 22士5%of the control to 51士7%(PLO. 01).The a,A-AR mRNA level was increased，
a,B-AR mRNA level decreased while a,D-AR unchanged. The results suggest that in cardiac insufficient rat hearts the subtype composition is altered as shown by increased a,A-AR，decreased a1B-AR and unchanged a,D-AR，although the total amount of a,-AR isunchanged.

Key words: cardiac insufficiency;Heart;#alpha#_(1)adrenergic receptor;receptor subtypes

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引用本文：

许开明, 张幼怡, 曲鹏, 张萍, 田斌, 韩启德. 心功能不全大鼠心脏#alpha#_(l)一肾上腺素受体亚型的变化[J]. 生理学报 1996; 48 (5): .

XU KAI-MING, ZHANG YOU-YI , QU PENG, ZHANG PING, Tian Bin, HAN QI-DE (CHIDE HAN). ALTERATION OF CARDIAC #alpha#_(l)-ADRENERGIC RECEPTOR SUBTYPES IN CARDIAC INSUFFICIENT RAT. Acta Physiol Sin 1996; 48 (5): (in Chinese with English abstract).