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胃粘膜血流量对大鼠胃粘膜适应性细胞保护作用的影响

阎长栋, 顾洛, 田苏平, 陈启盛, 戴义隆, 李栋生

南京医科大学生理教研室，南京 210029

摘要

采用氢气清除法测定胃粘膜血流量(gastric mucosal blood flow, GMBF)，观察大鼠胃内灌注低浓度盐酸酒精(0. 15 mol /L HCl-!-15 0 o EtOH)后灌注高浓度盐酸酒精(0. 6 mol/L HCl+15ooEtOll )对GMBF和胃粘膜损害的影响，以及GMBF在适应性细胞保护中的作用。结果如下:(1)先用低浓度盐酸酒精作为弱刺激灌注随之以高浓度盐酸酒精作为强刺激灌注，引起胃粘膜适应性保护现象，它表现为:大体损伤和损伤深度分别比单独强刺激组减少47.09%和44. 57 %(P<0. 01,P<0. 105);与此同时，GMBF亦出现适应性增强反应，即比单独强刺激组增加28. 02 %(P<0. 05); (2)近胃动脉灌注血管加压素后，GMBF的适应性增强反应被阻断，对胃粘膜的适应性保护作用也消失;(3)静脉注射消炎痛后，GMBF的适应性增强反应被阻断，对胃粘膜的适应性细胞保护作用消夫，粘膜损伤程度加重。上述结果表明:低浓度盐酸酒精作为弱刺激引起的适应性细胞保护作用与GMBF增加反应增强有关;减弱或阻断这一增强效应可使适应性细胞保护作用消失;内源性前列腺素参与适应性细胞保护作用可能部分与GMBF增加有关。

关键词： 胃粘膜血流量; 适应性细胞保护; 胃粘膜损害; 血管加压素; 消炎痛; 氢气清除率

EFFECTS OF GASTRIC MUCOSAL BLOOD FLOW (GMBF) ON THE ROLE OF ADAPTIVE CYTOPROTECTION OF RAT GASTRIC MUCOSA

YAN CHANG-DONG, GU LUO, TIAN SU-PING, CHEN QI-SHENG, DAI YI-LONG , LI DONG-SHENG

(Department of Physiology, Nan)ing Medical University，Nanjing 210029)

Abstract

By the use of hydrogen gas clearance technique，we have investigated the role of GMBF in the adaptive cytoprotection induced by intragastric perfusion with low concentration prior to high concentration of HCl plus ethanol. The results were as follows:(1)intragastric perfusion with low concentration prior to high concentration of HCl plus ethanol led to an adaptive cytoprotection，i. e.，the gross and the deep damage were decreased by 47. 09%and 44.57%respectively，as compared with those caused by high concentration of HCl plus ethanol alone;correspondingly，GMBF also showed an adaptive hyperemic response，i. e.，GMBF was increased by 28. 02%as compared with that due to high concentration alone;(2)close arterial infusion of vasopressin
blocked the adaptive hyperemic response and abolished the adaptive cytoprotection;(3)intravenous indomethacin reduced the basal GMBF，and abolished both the adaptive hy}peremic response and ytoprotection;furthermore，the gross and deep damage were aggravated compared with that caused by high concentration alone. The results showed that the adaptive hyperemic response of gastric mucosa was involved in the adaptive cytoprotection and suggested that the adaptive cytoprotection of endogenous prostaglandin might be partially related to the increase of GMBF.

Key words: gastric mucosal blood flow;adaptive cytoprotection;gastric lesions;vasopressin; indomethacin;hydrogen gas clearance

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引用本文：

阎长栋, 顾洛, 田苏平, 陈启盛, 戴义隆, 李栋生. 胃粘膜血流量对大鼠胃粘膜适应性细胞保护作用的影响[J]. 生理学报 1996; 48 (5): .

YAN CHANG-DONG, GU LUO, TIAN SU-PING, CHEN QI-SHENG, DAI YI-LONG , LI DONG-SHENG. EFFECTS OF GASTRIC MUCOSAL BLOOD FLOW (GMBF) ON THE ROLE OF ADAPTIVE CYTOPROTECTION OF RAT GASTRIC MUCOSA . Acta Physiol Sin 1996; 48 (5): (in Chinese with English abstract).