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热暴露大鼠心肌细胞钙稳态及其调节机制的研究

杨晓昊, 李文选, 程素琦, 陈西京

(天津卫生学环境医学研究所，天津300050)

摘要

观察了离体成年大鼠心室肌肌质网、线粒体Ca~(2十)一ATP酶活性和总钙含量以及原代培
养乳鼠心肌细胞~(45)Ca摄取、活性钙调素相对含量、胞浆内游离钙浓度在不同温度热暴露40 min后的变化。结果表明:热暴露后，心肌匀浆、肌质网及线粒体中的Ca~(2十)一ATP酶活性有所下降，心肌肌质网及线粒体中的总钙含量亦有降低趋势，心肌细胞活性钙调素相对含量显著下降，ash摄取量及胞浆内游离钙浓度显著升高。提示:热暴露后心肌细胞钙超载是导致机体心功能损害的重要原因之一。

关键词： 热基异; 心肌细胞; 钙稳态

MECHANISM OF INTRACELLULAR CALCIUM HOMEOSTASIS IN HEAT-EXPOSED CARDIOMYOCYTES OF RATS

YANG XIAO-HAO, LI WEN-XUAN, CHENG SU-QI, CHEN XI-JING

(Institute of Health and Environmental Medicine, Tianjin 300050)

Abstract

The activity of Cat+-ATPase and calcium content were measured in sarcoplasmic reticulum (SR) and mitochrondria of myocardium from 40 adult female Wistar rats after exposure to 3 7℃，39℃，41'C and 4 3℃for 40 min respectively in vitro. The 45Ca uptake，intracellular free CaZ+ concentration(ECaZ+]，)and the relatively active calmodulin content were also observed in cultured cardiomyocytes from 120 neonatal Wistar rats under the same condition of heat exposure. The results showed that(1)the activity of Caz+-ATPase of SR and mitochrondria of myocardium decreased after heat exposure，(2) the calcium content of SR and mitochrondria also showed a tendency of decrease with increase of exposure temperature，(3) the "Ca uptake and mean[CaZ+];
increased，whereas the calmodulin content decreased. It is suggested that disturbances of intracellular calcium homeostasis may be responsible for cardiac functional disorder after heat exposure.

Key words: :heat exposure;Cardiomyocyte;intracellular calcium

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引用本文：

杨晓昊, 李文选, 程素琦, 陈西京. 热暴露大鼠心肌细胞钙稳态及其调节机制的研究[J]. 生理学报 1996; 48 (5): .

YANG XIAO-HAO, LI WEN-XUAN, CHENG SU-QI, CHEN XI-JING. MECHANISM OF INTRACELLULAR CALCIUM HOMEOSTASIS IN HEAT-EXPOSED CARDIOMYOCYTES OF RATS. Acta Physiol Sin 1996; 48 (5): (in Chinese with English abstract).